Methods in Neurotransmitter and Neuropeptide Research

1st Edition, Volume 11 - January 4, 1994
Editors: S.H. Parvez, M. Naoi, T. Nagatsu
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 9 0 2 2 - 5

This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international… Read more

Methods in Neurotransmitter and Neuropeptide Research

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This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international specialists. The biochemical methods include liquid chromatography/electrochemistry, liquid chromatography/fluorescence and chemiluminescence detection, gas chromatography or liquid chromatography with mass spectrometry, and immunoassays.

Morphological and physiological methods are also described, including immunohistochemistry, in situ hybridization, in vivo microdialysis, electrophysiological analysis, microwave irradiation, electrophysiological analysis, micro-anatomical dissection, peptide sequence analysis, quantitative autoradiographic analysis, and isolated CNS preparations. The neurotransmitters and neuropeptides include catecholamines, indoleamines, histamine, polyamines, and various neuropeptides, and their related enzymes. The book is the most comprehensive, advanced and versatile volume on the biochemical, morphological and physiological analysis of neurotransmitters and neuropeptides.

PART 1. 1. Multi-electrode detection system for determination of neurotransmitters (M. Naoi, W. Maruyama, I.N. Acworth, D. Nakahara, H. Parvez). 2. Determination of biogenic amines, their metabolites, and other neurochemicals by liquid chromatography/electrochemistry (C.T. Duda, P.T. Kissinger). 3. High performance liquid chromatography-fluorometric or chemiluminometric detection of neurotransmitters and their metabolites (K. Imai, H. Watanabe). 4. Combined gas chromatography mass spectrometry for the analysis of biogenic amines (F. Karoum, S. Chrapusta, M. Egan). 5. Immunochemistry and in situ hybridization of catecholamine-synthesizing enzymes and the related neurotransmitters (I. Nagatsu, K. Yamada, M. Sakai, N. Karasawa). 6. Electron microscopic exploration of synaptic organization (D.G. Jones). 7. In vivo microdialysis of neurotransmitters and their metabolites (D. Nakahara, N. Ozaki, T. Nagatsu). 8. Electrophysiological analysis of sympathetic function (H. Togashi, M. Yoshioka, H. Saito). 9. Current status of rapid enzyme inactivation through the use of microwave irradiation (Y. Maruyama, Y. Ikarashi, C.L. Blank, W.B. Stavinoha). 10. Precise micro- and macro-anatomical dissection of the rat brain and the investigation of neuroendocrine function (H. Parvez, S. Parvez, M. Naoi). 11. Analysis of enzymes in catecholamine biosynthesis (K. Kojima, H. Parvez, S. Parvez, T. Nagatsu). 12. Catechol-O-methyltransferase (C.R. Creveling). Subject index. Part 2. 1. Immunoassays of catecholamines and their metabolites (M. Yoshioka). 2. Assay of serotonin, related metabolites and tryptophan hydroxylase (C.J. Schmidt, D.M. Kuhn, W. Lovenberg). 3. Determination of the monoamine oxidases (K.F. Tipton, M.B.H. Youdim). 4. Histamine and its related metabolites (A. Yamatodani, H. Wada). 5. Histamine and its related enzymes (H. Hayashi, T. Watanabe). 6. Histamine receptors (H. Fukui, K. Yanai). 7. Assay methods for polyamines (K. Fujita, T. Nagatsu and K. Shimpo). 8. Quantitative methods for trace amines (D.A. Durden, B.A. Davis). 9. Analysis of tetrahydroisoquinolines in the brain by gas chromatography/mass spectrometry (T. Niwa, M. Naoi, M. Yoshida, T. Nagatsu). 10. Ultrasensitive enzyme immunoassay for peptides (S. Hashida, K. Tanaka, N. Yamamoto, E. Ishikawa). 11. Analysis of neuropeptides by high performance liquid chromatography (K. Kojima, Y. Suzuki, H. Parvez, S. Parvez, T. Nagatsu). 12. Peptide sequence analysis (K. Takio, K. Titani). 13. Neuropeptide-degrading peptidase (F. Checler). 14. Peptidylglycine &agr;-amidating enzyme (K. Mizuno, H. Matsuo). 15. Quantative autoradiographic analysis of brain atrial natriuretic
peptide receptors (S. Zorad, J.M. Saavedra). 16. Isolated CNS preparations for studies on substance P and other neurotransmitters (H. Suzuki and M. Otsuka). Subject index.