Bioluminescence and Chemiluminescence

Basic Chemistry and Analytical Applications

1st Edition - April 28, 1981
Editors: Marlene A. Deluca, William D. McElroy
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 7 3 7 3 - 0

Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications is a compendium of papers presented at the second International Symposium on Analytical… Read more

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Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications is a compendium of papers presented at the second International Symposium on Analytical Applications of Bioluminescene and Chemiluminescence in San Diego, California on August 26-28, 1980. Part I deals with chemilunescence and excited states including topics on the spectroscopy of the solvent cage — generation and characteristics of the excited state and the three features of chemi- and bioluminescence. Part II deals with events prior to producing luminol-dependent chemiluminescence; this part also examines the effects of stimulants on membrane potential. Part III discusses bacterial bioluminescence and analyzes the properties of a lumazine protein from a bioluminescent bacterium. This part also analyzes accessory enzymes responsible for such bacterial bioluminescence. Part IV examines the chemistry of firefly bioluminescence and presents the formula of three reactions catalyzed by firefly luciferase. Part V analyzes bioluminescence from other sources such as the earthworm and land snails. Part VI discusses the applications of bioluminescence in clinical chemistry, soil science, and marine biology. Part VII deals with the future uses of bioluminescence, while Part VIII is an abstract of other papers dealing with this subject. This collection can be helpful for biologists, zoologists, micro-biologists, marine biologists, and researchers dealing with bio-chemistry and related disciplines.

Frontispiece

Contributors

Preface

I. Chemiluminescence and Excited States

Spectroscopy of the Solvent Cage. Generation and Characteristics of the Excited States

Chemiluminescence

Chemiluminescence of Oxygenase Reactions

Activated Chemiluminescence of Dimethyldioxetanone

Discussion

II. Luminol-Dependent Chemiluminescence

Preliminary Events Leading to the Production of Luminol-Dependent Chemiluminescence by Human Granulocytes

Chemiluminescence Immunoassay for Anti-Human IgG

Lucigenin Chemiluminescence: A New Approach to the Study of Polymorphonuclear Leukocyte Redox Activity

Measurement of Opsonic Activity of Serum by Granulocyte Chemiluminescence

On the Question of Singlet Oxygen Production in Polymorphonuclear Leucocytes

Discussion

III. Bacterial Bioluminescence

Fluorescence Properties of Luciferase Peroxyflavins Prepared with Iso-FMN and 2-Thio FMN

Properties of a Lumazine Protein from the Bioluminescent Bacterium Photobacterium Phosphoreum

Purification, Identification, and Synthesis of Photobacterium Fischeri Autoinducer

Active Center Studies on Bacterial Luciferase: Evidence That the Reactive Cysteinyl Residue Is within the Protease-Labile Region of the a Subunit

Bacterial Bioluminescence: Accessory Enzymes

Bioluminescence Test for Mutagenic Agents

A Thermodynamic Explanation for the Kinetic Differences Observed Using Different Chain Length Aldehydes in the in Vitro Bacterial Bioluminescent Reaction

Active Center Studies on Bacterial Luciferase: Modification with Methyl Methanethiolsulfonate

Isolation and Reaction Product Characterization of an Oxygenated Bacterial Luciferase Intermediate Formed with a Flavin Analog

Discussion

IV. Firefly Bioluminescence

The Chemistry and Applications of Firefly Luminescence

Applications of Firefly Luminescence

Dynamic Measurement of ATP with Firefly Luciferase Bioluminescence

Preliminary Report on the Pink-Colored Cypridina Luciferase, A Natural Model of the Luciferin-Luciferase Complex

Standard Assay for Total Creatine Kinase and the MB-Isoenzyme in Human Serum with Firefly Luciferase

Discussion

V. Renilla, Aequorea, Earthworm, and Land Snail Bioluminescence

Renilla and Aequorea Bioluminescence

Properties of the Coelenterate Green-Fluorescent Proteins

Applications of Aequorin

Earthworm Bioluminescence

Luminescence Activity of the Land Snail Quantula Striata

Discussion

VI. Applications of Bioluminescence

Overview of Applications of Bioluminescence

Immunoassays Monitored by Chemilumigenic Labels

Aspects on the Potentialities of Bioluminescence Assay in Cell Biology and Microphysiology

Discussion

VII. Summary and Future Applications of Luminescence

Overview

VIII. Abstracts

A. Chemiluminescence and Related Assays

A Preliminary Study of the Measurement of Urinary Pregnanediol-3α- Glucuronide by a Solid-Phase Chemiluminescence Immunoassay

Chemiluminescent Reactions of Alcohols and Aldehydes

Defects Associated with the Transmembrane Potential of Granulocytes in Chronic Granulomatous Disease

Room Temperature Phosphorescence of Some Pharmaceutical Important Imidazoles

An Oxygen Probe Based on Tetrakis Alkyl Aminoethylene Chemiluminescence

An Immunoassay for Urinary Estriol-16a Glucuronide Based on Antibody-Enhanced Chemiluminescence

Assay of Gonadal Steroids Based on Antibody-Enhanced Chemiluminescence

Dissociative and Non-Dissociative Electron Transfer Mechanisms in Bioluminescence and Chemiluminescence

Site-Specific Chemiluminescent Probes Used in the Analysis of pH, Peroxidation, and Free Radicals Internally in Metabolically Active Organelles

Differential Effects of Bacterial and Viral Infections of Granulocyte Chem i luminescence (CL)

Archadonate-Based Chemiluminescence in Human Granulocytes and Platelets Using the Monolight 301 (Drug Studies)

Chemiluminescence Derived from 4a-(Alkylperoxy) Flavins

B. Bacterial Bioluminescence and Applications

The Use of Bentonite Clays to Increase the Speed and Yield of Harvest of Luminous Bacteria by Continuous Flow Centrifugation

The Red Absorbing Flavin Species in the Reaction of Bacterial Luciferase with FMNH2 and O2

Bacterial Bioluminescence: Applications to Entomology

The Use of the Bacterial Luminescent System for the Quantitative Determination of Dehydrogenases

Activity Coupling between Bacterial Luciferase and Flavin Adenine Dinucleotide-Dependent Salicylate Hydroxylase

C. Firefly Luciferase and Applications

Analytical Aspects of the Firefly Luciferase Reaction Kinetics

Bioluminescent Screening for Bacteriuria

Purification of Firefly Luciferase by Ammonium Sulphate Precipitation and Isoelectric Focusing

A Convenient Affinity Chromatography-Based Purification of Firefly Luciferase

Bioluminescence Microassay of Creatine-Kinase: A Comparison with a Spectrophotometric Method

Inhibitors of Firefly Luciferase in Clinical Urine Specimens

Use of a Mathematical Representation of the Time-Course of the Firefly Luciferase Light Reaction

Studies on the Sensitivity of ATP Determination Using Commercial Firefly Luciferase

D. General Bioluminescence and Chemiluminescence, Including Dinoflagellates, Worms and Fish

Chemiluminescence of a 6,7-Dihydroflavin and Some Related Pteridines

In Vivo Spectroscopy of a Bioluminescent Cell: Pyrocistis Lunula

The Counterlighting Hypothesis: In Situ Observations on Argyropelecus Hemigymnus

Biochemical Studies of the Bioluminescent Polynoid Worms

Red Fluorescence of Fish and Cephalopod Photophores

Oxygen-Dependent Streptococcus faecalis Chemiluminescence: The Importance of Metabolism and Medium Composition

Preliminary Ultrastructural Description of Coelomocytes of the Luminescent Oligochaete, Pontodrilus bermudensis (Annelida)

On the Bacterial-Associated Light Organ in Chlorophthalmus

Circular Polarization Observed in Bioluminescence

E. Application of Bioluminescence and Chemiluminescence to Clinical and Biological Problems

Advantages in Microprocessing Techniques in Bioluminescence Assays

Autolumography: Using Bioluminescence to "Stain" for Enzyme Activities Resolved on Polyacrylamide Gels

Bioenergetics of Insulin and Glucagon Producing Cells Evaluated in Bioluminescence Assays

Applications of the Photoprotein Obelin to the Measurement of Free Ca2+ in Cells

Chemiluminescence of Cytotoxic Macrophages

Measurements of Granulocyte and Platelet Chemiluminescence in Small Samples of Whole Blood

Flow Injection Analysis with Chemiluminescence Detection; Recent Advances and Clinical Applications

Ultrasensitive Assay of NAD/NADH with Firefly Luciferase

A Clinical Use of Solid Phase Sepharose-Isoluminol for Sensitive Thiol Assay of Serum Cholinesterase

Chemiluminescence Experiments on a Model of the Oligodynamic Activity of Silver

Chemiluminescent Immunoassay for the Detection of Virus/Antibody Aggregates

An Immunoassay for Plasma Cortisol Based on Chemiluminescence

Determination of Hemoglobin and Other Hemoproteins in Serum by Luminol Chemiluminescence

Bioluminescent Immunoassay: A New Enzyme-Linked Analytical Method for the Quantitation of Antigens

Chemiluminescent Labels in Immunoassay

Evaluation of Metabolic Rates Using Bioluminescence Analysis

1,2-Dioxetanes as Chemiluminescent Probes and Labels

Design of a Chemiluminescent and Bioluminescent Photometer

Bioluminescent Immunoassays: Use of Luciferase-Antigen Conjugates for Determination of Methotrexate and DNP

F. Instrumentation and Reagents

Six-Channel Luminescence Analyzer for Phagocytosis Applications

Utilization of Multi-Injection for Sample Processing and Automatic Internal Standardization (AIS) in Luminometry

Preliminary Report on the Mechanism of Bioluminescence in the Oceanic Squid Symplectoteuthis

A Novel Rapid-Mixing Apparatus for Studies of Chemiluminescent Reactions

Signals of Chemiluminescence Emitted by Spleen Cells and Bone Marrow Macrophages after Stimulations with Mitogens and Paniculate Substances

Chemiluminescence of Lucigenin in Micellar Systems

Pico-Zyme F and Pico-EX for Measuring Cellular ATP on a Pico-Lite Luminometer

High Quality Bacterial Luminescence Reagents for FMN Measurements

A Comparison of Commercial Firefly Luciferases

Luminescent Oscillations in Microaerobic Batch Cultures of Photobacterium Phosphoreum

Index of Authors

Subject Index