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Methods in Cilia and Flagella
1st Edition, Volume 127 - March 25, 2015
Editors: Renata Basto, Wallace F. Marshall
Language: English
Hardback ISBN:9780128024515
9 7 8 - 0 - 1 2 - 8 0 2 4 5 1 - 5
eBook ISBN:9780128026403
9 7 8 - 0 - 1 2 - 8 0 2 6 4 0 - 3
The goal of this book is to collect methods and protocols for studying cilia in a wide range of different cell types, so that researchers from many fields of biology can start ex…Read more
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The goal of this book is to collect methods and protocols for studying cilia in a wide range of different cell types, so that researchers from many fields of biology can start exploring the role of cilia in their own system.
Chapters are written by experts in the field
Cutting-edge material
Biological researchers at any level who want to learn methods for studying cilia in different cell types
Series Editors
Contributors
Preface
Chapter 1. Imaging centrosomes and cilia in the mouse kidney
Introduction
1. Methods
Conclusion
Chapter 2. Ependymal cell differentiation, from monociliated to multiciliated cells
2. Visualization of IFT and Acquisition of Time-Lapse Videos
3. Quantification of IFT
Conclusion
Chapter 24. Scanning and three-dimensional electron microscopy methods for the study of Trypanosoma brucei and Leishmania mexicana flagella
Introduction
1. Rationale
2. Methods
3. Materials
4. Discussion
Volumes in Series
Index
No. of pages: 614
Language: English
Edition: 1
Volume: 127
Published: March 25, 2015
Imprint: Academic Press
Hardback ISBN: 9780128024515
eBook ISBN: 9780128026403
RB
Renata Basto
Affiliations and expertise
Cell Biology Department, CNRS, Institut Curie, France
WM
Wallace F. Marshall
Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.
Affiliations and expertise
Department of Biochemistry & Biophysics, University of California San Fransisco at Mission Bay, USA