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Skin Tissue Engineering and Regenerative Medicine
1st Edition - January 14, 2016
Authors: Mohammad Albanna, James H Holmes IV
Language: English
Paperback ISBN:9780128016541
9 7 8 - 0 - 1 2 - 8 0 1 6 5 4 - 1
eBook ISBN:9780128017975
9 7 8 - 0 - 1 2 - 8 0 1 7 9 7 - 5
The skin is the largest human organ system. Loss of skin integrity due to injury or illness results in a substantial physiologic imbalance and ultimately in severe disability or de…Read more
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The skin is the largest human organ system. Loss of skin integrity due to injury or illness results in a substantial physiologic imbalance and ultimately in severe disability or death. From burn victims to surgical scars and plastic surgery, the therapies resulting from skin tissue engineering and regenerative medicine are important to a broad spectrum of patients.
Skin Tissue Engineering and Regenerative Medicine provides a translational link for biomedical researchers across fields to understand the inter-disciplinary approaches which expanded available therapies for patients and additional research collaboration. This work expands on the primary literature on the state of the art of cell therapies and biomaterials to review the most widely used surgical therapies for the specific clinical scenarios.
Explores cellular and molecular processes of wound healing, scar formation, and dermal repair
Includes examples of animal models for wound healing and translation to the clinical world
Presents the current state of, and clinical opportunities for, extracellular matrices, natural biomaterials, synthetic biomaterials, biologic skin substitutes, and adult and fetal stem and skin cells for skin regenerative therapies and wound management
Discusses new innovative approaches for wound healing including skin bioprinting and directed cellular therapies
Biomedical researchers in the fields of tissue engineering and regenerative medicine; stem cell researchers
Dedication
List of Contributors
Foreword
Chapter 1. Anatomy, Physiology, Histology, and Immunohistochemistry of Human Skin
Introduction
Skin Anatomy, Histology, and Physiology
Epidermis
Dermoepidermal Junction
Dermis
Hypodermis
Wound Healing and Immunohistochemistry
Chapter 2. Molecular and Cellular Biology of Wound Healing and Skin Regeneration
Introduction
Fibroproliferative Disorders of the Skin
Medical Therapies for Skin Regeneration
Future Directions
List of Abbreviations
Chapter 3. Tissue Processing and Staining for Histological Analyses
Introduction
Tissue Fixation
Tissue Processing and Embedding
Hematoxylin and Eosin Stain
Bright Field Microscopy
Immunofluorescence
Immunohistochemistry
Histochemical Stains
Conclusion
Chapter 4. Clinical Management of Wound Healing and Hypertrophic Scarring
Wound Healing and the Biomedical Burden of Its Dysfunction
Stages of Wound Healing
Pathologic Wound Healing
Chronic Wounds
Wound Healing Therapies
Fibroproliferative Disease
Scar Reduction Therapies
Regenerative Healing
Conclusion
List of Abbreviations
Chapter 5. Process Development and Manufacturing of Human and Animal Acellular Dermal Matrices
Introduction
Clinical Need
Development of ADMs
ADM Requirements
Processing Methodologies
Biological Responses to ADMs
Clinical Use
Summary
Chapter 6. Clinical Applications of Acellular Dermal Matrices in Reconstructive Surgery
Introduction
Animal Data
Clinical Applications
Conclusion/Future
Chapter 7. Advances in Acellular Extracellular Matrices (ECM) for Wound Healing
Introduction
Acellular Matrices
Manufacturing Process
Mode of Action
Matrix Application
Evidence of Acellular Matrices Use in Other Conditions
Conclusions
List of Acronyms and Abbreviations
Chapter 8. Natural Biomaterials for Skin Tissue Engineering
Introduction
Natural Biomaterials Found in ECM
Other Natural Biomaterials
Applications of Natural Biomaterials in STE
General Considerations on the Applicability of Biomaterials in STE
List of Acronyms and Abbreviations
Chapter 9. Synthetic Biomaterials for Skin Tissue Engineering
Introduction
Characteristics of the Ideal Skin Substitute
The Need for Synthetic Materials in Skin Substitutes
Development of Synthetic Skin Substitutes over Time
Selected Patents on “Artificial Skin”
Selected Examples of Commercially Available Skin Substitutes
Limitations of Available Skin Substitutes (Problems with “Off-the-Shelf” Skin Substitutes)
Selected Materials and Fabrication Methods
Future Prospects and Concluding Remarks
Chapter 10. Hybrid Biomaterials for Skin Tissue Engineering
Introduction
Skin: Structure and Function
Skin Injury
How Can We “Close” an Extensive Cutaneous Wound?
Why Do We Need Alternatives to the Skin Graft?
What Needs to Be Considered in Treating Deep Skin Injury/Loss?
An Introduction to Biodegradable Temporizing Matrices
An Introduction to In Vitro Dermo-epidermal (Composite) Cultured Skins
The Future
Chapter 11. Biologic Skin Substitutes
Introduction and Medical Needs
Biological Requirements and Current Alternatives
Deficiencies of Biologic Skin Substitutes
Regenerative Medicine and Mechanisms of Developmental Biology
Gene Therapy Approaches
Regulation of Skin Substitutes by the US Food and Drug Administration
Conclusions and Future Directions
List of Acronyms and Abbreviations
Glossary
Chapter 12. Wound Healing: A Comprehensive Wound Assessment and Treatment Approach
Wound Healing, Then and Now
Wound Healing Process
Factors Impacting Wound Healing
Work-Up of a Patient with a Nonhealing Wound
Common Wound Etiologies/Treatment
Future Directions
Chapter 13. Current Innovations for the Treatment of Chronic Wounds
Burden of Diabetic Foot Ulcers
Wound Healing
Cutaneous Microbiota
Standard of Care
Advanced Bioengineered Treatment Options
Next-Generation Skin Substitutes
Tapping Innate Antimicrobials
Cathelicidin, a Multifunctional HDP
A Promising Skin Tissue with Enhanced Cathelicidin Expression
Safety Features of Genetically Modified Skin Tissue
Designer Tissues for Specific Needs
Conclusions
Chapter 14. The Surgical Management of Burn Wounds
Epidemiology of Burn Injuries
Presentation of Burn Injuries
Resuscitation
Repair
Chapter 15. Advances in Isolation and Expansion of Human Cells for Clinical Applications
Introduction
Skin Harvesting
Cell Isolation
Cell Expansion
Clinical Application
Conclusion
Chapter 16. Cutaneous Applications of Stem Cells for Skin Tissue Engineering
Introduction: Stem Cells
Skin-Derived Stem Cells
Adult Stem Cells
Perinatal Tissues and Stem Cells
Summary and Future Applications of Stem Cells in Wound Healing
Chapter 17. Advances in Biopharmaceutical Agents and Growth Factors for Wound Healing and Scarring
Introduction
The Transforming Growth Factor-β Family
Other TGF-β-Based Approaches: Decorin and Mannose 6 Phosphate
Modulation of Smad3/Smad7 Signaling
Epidermal Growth Factor Family
Fibroblast Growth Factor Family
Platelet-Derived Growth Factor Family
Granulocyte Macrophage-Colony Stimulating Factor
Connective Tissue Growth Factor
Hepatocyte Growth Factor
Interleukin 10
Connexins
Other Approaches under Investigation for Scar Reduction
Discussion and Future Directions
List of Abbreviations
Chapter 18. Skin Models for Drug Development and Biopharmaceutical Industry
Outline of the Chapter
Introduction
Human Skin Models to Assess Pharmacologically Relevant Data
Realization of Disease Models
Experimental Parameters Influencing Readout
Gating Studies
Future Challenges
Conclusion
Chapter 19. Animal Models for Wound Healing
Introduction
Comparative Animal Models of Wound Healing
Animal Models of Acute Wounds
Animal Models of Scarless Wound Healing
Animal Models of Chronic Wounds
Conclusion
Chapter 20. Human Skin Bioprinting: Trajectory and Advances
Overview of Bioprinting
Skin Bioprinter Development
Skin Bioprinting
Conclusion
Chapter 21. Translational Research of Skin Substitutes and Wound Healing Products
Translational Research and the Commercialization Pathway
Target Product Profile and Regulatory Path
Economic Challenges and Strategic Development Framework
Future Translational Needs
Future Directions
Index
No. of pages: 466
Language: English
Edition: 1
Published: January 14, 2016
Imprint: Academic Press
Paperback ISBN: 9780128016541
eBook ISBN: 9780128017975
MA
Mohammad Albanna
Dr. Albanna is currently the R&D Projects Lead and supervisor of the R&D department at COOK General BioTechnology, LLC, a COOK Medical company. Dr. Albanna was the team leader of skin Bioprinting clinical research programs at Wake Forest Institute for Regenerative Medicine (WFIRM) working on utilizing autologous and allogeneic skin and stem cells for developing dermal/epidermal skin substitutes. Dr. Albanna expedited the transition of multiple skin bioprinting projects from bench-top into clinics through development of preclinical models for wound healing and skin regeneration, protocols for large scale expansion of skin and stem cells for clinical use. Dr. Albanna has several years of expertise in product development of tissue-engineered products including skin wound healing products. He is author or the co-author of several patents and publications including book chapters and peer-reviewed journals in esteemed journals in the field of tissue engineering and regenerative medicine including two recent accepted book chapters on biomaterials for skin regeneration and acellular dermis matrices for skin regeneration and surgical reconstruction to be published in 2014 in Encyclopedia of Biomedical Polymers and Polymeric Biomaterials along with Dr. Holmes as a senior author.
Affiliations and expertise
Adjunct Assistant Professor of General Surgery, Wake Forest University School of Medicine, Winston-Salem, NC, USA; Sr. R&D Manager, Pinnacle Transplant Technologies, Phoenix, AZ, USA
JH
James H Holmes IV
Dr. Holmes is an Associate Professor of Surgery and the Director of Wake Forest Baptist Medical Center (WFBMC) Burn Center. He is the current Chair of the American Burn Association (ABA) Southern Region. He is acting as the leading principal investigator for multiple burn clinical trials and is the current Chair of the ABA Multicenter Trials Group Burn Science Advisory Panel. He is co-author of an ABA white paper on the medical practice of treatment of wounds. He is author of many peer-reviewed journal papers and book chapters in the field of burns and wound healing.
Affiliations and expertise
Associate Professor of Surgery, Wake Forest University School of Medicine and Director, Wake Forest Baptist Medical Center (WFBMC) Burn Center, Winston-Salem, NC, USA
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