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Hydrogen Sulfide in Redox Biology Part B
1st Edition, Volume 555 - March 3, 2015
Editors: Enrique Cadenas, Lester Packer
Language: English
Hardback ISBN:9780128015117
9 7 8 - 0 - 1 2 - 8 0 1 5 1 1 - 7
eBook ISBN:9780128016220
9 7 8 - 0 - 1 2 - 8 0 1 6 2 2 - 0
These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide…Read more
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These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant.
Continues the legacy of this premier serial with quality chapters on hydrogen sulfide research authored by leaders in the field
Covers conventional and new hydrogen sulfide detection methods
Covers the pharmacological activity of hydrogen sulfide donors
Contains chapters on important topics on hydrogen sulfide modulation of cell signaling and transcriptional pathways, and the role of hydrogen sulfide in the cardiovascular and nervous systems and in inflammation
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.
Preface
Section I: The Redox Biochemistry of Hydrogen Sulfide
Chapter One: Investigating the Role of H2S in 4-HNE Scavenging
Abstract
1 Introduction
2 Experimental Compounds and Considerations
3 Conclusions and Perspectives
Acknowledgment
Chapter Two: Inhalation Exposure Model of Hydrogen Sulfide (H2S)-Induced Hypometabolism in the Male Sprague-Dawley Rat
Abstract
1 Introduction
2 Exposure Protocol for H2S-Induced Hypometabolism in Rats
3 Other Considerations for H2S Exposure Studies
4 Summary
Acknowledgments
Section II: Mechanisms of H2S Cell Signaling and Transcriptional Pathways
Chapter Three: Use of the “Tag-Switch” Method for the Detection of Protein S-Sulfhydration
Abstract
1 Introduction
2 The Design of “Tag-Switch” Method
3 Chemistry Validation Using Small-Molecule Substrates
4 “Tag-Switch” Assay on Bovine Serum Albumin and GAPDH as Model Proteins
5 “Tag-Switch” Assay for the Detection on Intracellular Protein Persulfides
6 “Tag-Switch” Assay for the Detection of Intracellular S-Sulfhydration by Fluorescence Microscopy
7 Conclusions
Acknowledgments
Chapter Four: Real-Time Assays for Monitoring the Influence of Sulfide and Sulfane Sulfur Species on Protein Thiol Redox States
Abstract
1 Introduction
2 PTEN Activity Assay
3 roGFP2 Redox Assay
4 Application of “H2S Donors” and Polysulfides
5 Quantitation of Sulfane Sulfur by Cold Cyanolysis
6 Elimination of Sulfane Sulfur by Cold Cyanolysis
Acknowledgments
Chapter Five: Protein Sulfhydration
Abstract
1 Introduction
2 Detection of Sulfhydration Using the Modified Biotin Switch Assay
3 Detection of Sulfhydration Using the Maleimide Assay
4 Summary
Acknowledgments
Section III: H2S in Cell Signaling in the Cardiovascular and Nervous System and Inflammatory Processes
Chapter Six: Intravital Microscopic Methods to Evaluate Anti-inflammatory Effects and Signaling Mechanisms Evoked by Hydrogen Sulfide
Abstract
1 Introduction
2 Molecular Determinants of Neutrophil/Endothelial Cell Adhesive Interactions
3 Intravital Microscopic Approaches to Study Leukocyte/Endothelial Cell Adhesive Interactions
4 Assessing Leukocyte Rolling, Adhesion, and Emigration in the Intact Microcirculation
5 Detection of Chemokine and Adhesion Molecule Expression using Intravital Microscopy
6 Intravital Microscopic Methods to Assess Changes in Microvascular Permeability
7 Assessment of Reactive Oxygen Species Generation Using Intravital Microscopy
8 Fluorescence Detection of Cell Injury using Intravital Microscopy
9 Perfused Capillary Density Assessment with Intravital Microscopy
10 Acute and Preconditioning-Induced Anti-inflammatory Actions of Hydrogen Sulfide: Assessment Using Intravital Microscopy
11 Conclusion and Perspectives
Acknowledgment
Chapter Seven: Attenuation of Inflammatory Responses by Hydrogen Sulfide (H2S) in Ischemia/Reperfusion Injury
Abstract
1 Introduction
2 Ischemia–Reperfusion Injury
3 Central Nervous System
4 Respiratory System
5 Cardiovascular System
6 Gastrointestinal System
7 Hepatobiliary System
8 Renal System
9 Musculoskeletal
10 Summary
Chapter Eight: CD47-Dependent Regulation of H2S Biosynthesis and Signaling in T Cells
Abstract
1 Introduction
2 Regulation of H2S Biosynthesis in T Cells
3 Catabolism of H2S
4 Regulation of T cell Activation by H2S Signaling
5 Autocrine and Paracrine Roles of H2S in T cell Activation
6 Role of H2S in the Cytoskeleton
7 T Cell Regulation by TSP1/CD47 Signaling
8 H2S Regulation of Leukocyte Adhesion
9 Role of H2S in Diseases Associated with Altered T cell Immunity
10 Future Prospective
Acknowledgments
Chapter Nine: Anti-inflammatory and Cytoprotective Properties of Hydrogen Sulfide
Abstract
1 Introduction
2 Enzymatic Synthesis of H2S
3 Healing and Resolution of Inflammation
4 Mechanisms of Anti-inflammatory Effects of H2S
5 Effects of H2S on Visceral Pain
6 Cytoprotective Actions of H2S
7 Therapeutic Applications of H2S-Releasing Drugs
Acknowledgments
Chapter Ten: H2S and Substance P in Inflammation
Abstract
1 Introduction
2 Disease Models Used to Study the Role of H2S and Substance P
3 H2S and Substance P—What Are They Doing Together?
4 Summary
Acknowledgments
Chapter Eleven: Role of Hydrogen Sulfide in Brain Synaptic Remodeling
Abstract
1 Introduction
2 Pharmacological and Physiological Effect of H2S
3 Effect of H2S on the CNS
4 Effect of H2S on Brain Cells (Astrocyte, Microglia, and Oligodendrocyte)
5 Synapse
6 Glia and Neurons Interactions
7 Effect of H2S on Neuronal Redox Stress
8 Effect of H2S on Glutamate Neurotransmission
9 Effect of H2S on NMDA Receptor Regulation
10 Effect of H2S on GABA-Mediated Neurotransmission
11 Effect of H2S on Calmodulin Kinase
12 Conclusion
Conflict of Interest
Acknowledgment
Section IV: H2S in Plants
Chapter Twelve: Detection of Thiol Modifications by Hydrogen Sulfide
Abstract
1 Introduction
2 Hydrogen Sulfide Acts as a Signal in Cells
3 Modification of Thiols by Signaling Molecules
4 Identification of Modified Thiols by Other Methods
5 Experimental Protocols
6 Caenorhabditis elegans as a Model Organism
7 Growth of C. elegans
8 Treatment of Samples with H2S
9 Estimation of Toxicity of H2S Compounds
10 Treatment of Samples with Thiol Tag
11 Isolation and Analysis of Modified Proteins
12 Estimation of Protein Concentrations in Samples
13 Further Analysis and Identification of Modified Proteins
14 Concluding Remarks
Chapter Thirteen: Analysis of Some Enzymes Activities of Hydrogen Sulfide Metabolism in Plants
Abstract
1 Theory
2 Equipment
3 Materials
4 Protocol 1
5 Step 1: Analyze of l-/d-Cysteine Desulfhydrase Activity
6 Protocol 2
7 Step 1: Analyze of Sulfite Reductase Activity
8 Protocol 3
9 Step 1: Analyze of β-Cyano-l-Alanine Synthase Activity
10 Protocol 4
11 Step 1: Analyze of l-Cysteine Synthase Activity
Acknowledgment
Chapter Fourteen: Sulfide Detoxification in Plant Mitochondria
Abstract
1 Introduction
2 Methods
3 Summary
Acknowledgments
Section V: Molecular Hydrogen
Chapter Fifteen: Molecular Hydrogen as a Novel Antioxidant: Overview of the Advantages of Hydrogen for Medical Applications
Abstract
1 Introduction
2 Comparison of H2 with Other Medical Gasses
3 Oxidative Stress as Pathogenic Sources
4 Physiological Roles of H2O2
5 Measurement of H2 Gas Concentration
6 Advantages of Hydrogen in Medical Applications
7 Methods of Ingesting Molecular Hydrogen
8 Medical Effects of H2
9 Possible Molecular Mechanisms Underlying Various Effects of Molecular Hydrogen
10 Unresolved Questions and Closing Remarks
Author Index
Subject Index
No. of pages: 370
Language: English
Edition: 1
Volume: 555
Published: March 3, 2015
Imprint: Academic Press
Hardback ISBN: 9780128015117
eBook ISBN: 9780128016220
EC
Enrique Cadenas
ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases.
Affiliations and expertise
Pharmacology & Pharmaceutical Sciences, School of Pharmacy, University of Southern California, USA
LP
Lester Packer
Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California.
Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California.
He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology:
Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.
Affiliations and expertise
Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA
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