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Forensic DNA Biology
A Laboratory Manual
- 1st Edition - August 3, 2012
- Author: Kelly M. Elkins
- Language: English
- Other ISBN:9 7 8 - 0 - 1 2 - 3 9 4 5 8 5 - 3
- eBook ISBN:9 7 8 - 0 - 1 2 - 3 9 4 8 3 3 - 5
DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biolo… Read more
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Request a sales quoteDNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies will be used throughout.
- Provides practical step-by-step instruction on how to perform forensic DNA analysis
- Includes analysis of hair, presumptive testing of body fluids, human DNA typing and statistics
- Covers techniques such as pipetting, microscopy and DNA extraction
- Pre- and post-lab exercises and questions assist the reader in learning the material
- Report writing templates assure the reader learns real world crime lab procedure
Upper-level, undergraduate or graduate students majoring in forensic science, chemistry or biology, academia and newly practicing forensic scientists
Acknowledgements
About the Author
Welcome
Forensic DNA Biology: An Introduction
Biology Overview
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Short Tandem Repeats
Single Nucleotide Polymorphisms
Mitochondrial DNA
Known versus Questioned Samples
Why Study Forensic DNA Biology?
Laboratory Safety
Rules for a Safe Lab Environment
Avoiding Contamination Issues
Chapter 1. Pipetting
Objective
Safety
Materials
Background
Procedure
Questions
Graphing the Data Using Microsoft Excel (2003)
Equations
REFERENCES
Chapter 2. Serology
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 3. Sampling Biological Evidence for DNA Extraction
Objective
Safety
Materials
Background
Procedure: DNA Collection and Packaging
Questions
REFERENCES
Chapter 4. DNA Extraction
Objective
Safety
Materials
Recipes for Buffer and Solution Preparation
Background
Procedure
Question
REFERENCES
Chapter 5. Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCE
Chapter 6. Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 7. Determination of DNA Quantity by Fluorescence Spectroscopy
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 8. Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 9. Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico)
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 10. Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 11. Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 12. Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex Kit
Objective
Safety
Materials
Background
Procedure
Question
REFERENCES
Chapter 13. Computing Random Match Probability from DNA Profile Data Using Population Databases
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 14. Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 15. Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 16. Ribonucleic Acid (RNA) Extraction
Objective
Safety
Materials
Background
Procedure
Question
REFERENCES
Chapter 17. Y-STR Polymerase Chain Reaction (PCR) Deoxyribonucleic acid (DNA) Amplification and Typing
Objective
Safety
Materials
Background
Procedure
Question
REFERENCES
Chapter 18. Human Genetic Analysis: Paternity or Missing Persons Cases and Statistics
Objective
Safety
Materials
Background
Procedure
REFERENCES
Chapter 19. Low Copy Number Stochastic Results
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 20. Using in Silico Methods to Construct a Short-Tandem Repeat (STR) Deoxyribonucleic Acid (DNA) Sequence for Cloning
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCE
Chapter 21. Deoxyribonucleic Acid (DNA) Extraction from Botanical Material and Polymerase Chain Reaction (PCR) Amplification
Objective
Safety
Materials
Background
Procedure
Questions
REFERENCES
Chapter 22. Social, Ethical, and Regulatory Concerns
REFERENCES
Selected Forensic DNA Biology Case Studies
Index
- No. of pages: 224
- Language: English
- Edition: 1
- Published: August 3, 2012
- Imprint: Academic Press
- Other ISBN: 9780123945853
- eBook ISBN: 9780123948335
KE
Kelly M. Elkins
Dr. Elkins is Assistant Professor of Chemistry at Towson University where she teaches in the Masters of Science in Forensic Chemistry Program. Previously, she was the Director of Forensic Science and Assistant Professor of Chemistry at Metropolitan State College of Denver. She has her Bachelor of Science in Biology, Bachelor of Arts in Chemistry and her PhD in Chemistry with an emphasis on Biochemistry. Focused on the Forensic DNA field, she has developed a network of contacts in crime laboratories throughout Colorado and the Four Corners region and looks forward to extending her network in the Baltimore/DC area.
Affiliations and expertise
Assistant Professor of Chemistry, Chemistry Department and Forensic Chemistry Program, Towson University, Towson, Maryland, USARead Forensic DNA Biology on ScienceDirect