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Modern Approaches to Animal Cell Technology
1st Edition - January 1, 1987
Editors: R.E. Spier, J.B. Griffiths
Language: English
eBook ISBN:9781483145099
9 7 8 - 1 - 4 8 3 1 - 4 5 0 9 - 9
Modern Approaches to Animal Cell Technology is a collection of papers presented at the 1987 joint European Society for Animal Cell Technology-OHOLO conference held in Tiberias,…Read more
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Modern Approaches to Animal Cell Technology is a collection of papers presented at the 1987 joint European Society for Animal Cell Technology-OHOLO conference held in Tiberias, Israel. Contributors explore modern approaches to animal cell technology and discuss the construction of the animal cell substrate, the physiology of those cells in a bioreactor type of environment, and the ways in which different products can be made from animal cells in culture and tested. This book is comprised of 59 chapters divided into nine sections and begins by outlining the history of issues and decisions that were made regarding the acceptability of various cell substrates, along with the use of continuous cell lines in biotechnology. The next chapter explores the feasibility, reproducibility, and the sensitivity of the dot-blot filter hybridization test to detect minute amounts of residual cellular DNA. The reader is then introduced to cells and cell lines such as monoclonal antibodies; cell growth and development; physiology of cells; and the use of bioreactors for culturing animal cells. Downstream unit processes, vaccines, immune system products, and toxicity testing with animal cells are also considered. This monograph will be a valuable resource for animal-cell technologists, biotechnologists, and microbiologists.
1. The Liberation of Animal Cells: Psychology of Changing Attitudes
2. Cellular DNA Biologicals: Significance and Quantitation
Section 1 New Cells and Cell Lines
3. Human Monoclonal Antibodies - Their Potential, Problems and Prospects
4. Production of Cancer Specific Monoclonal aAtibodies with Human-Human Hybridomas and Their Serum-free, High density, Perfusion Culture
5. Establishment of a Human Hybridoma Secreting Monoclonal Antibodies toward Rabies Virus
6. The Production of Bovine and Ovine Monoclonal Antibodies to Gonadal Steroids: The Use of Interspecific Hybridomas
7. The Immortalization of Differentiated Mammalian Cells