Immobilized Cells: Basics and Applications

Immobilized Cells: Basics and Applications, 1st Edition

Immobilized Cells: Basics and Applications, 1st Edition,R.M. Buitelaar,C. Bucke,J. Tramper,R.H. Wijffels,ISBN9780080534473

Buitelaar   &   Bucke   &   Tramper   &   Wijffels   

Elsevier Science



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This publication contains full papers of both oral and poster presentations of the symposium "Immobilized Cells: Basics and Applications" that was held in Noordwijkerhout, The Netherlands, 26-29 November 1995. This volume covers recent developments in the field of immobilization e.g.: new support materials, characterization of support materials, kinetic characterizations, dynamic modelling, bioreactor types, scale up and applications are also given. Applications in the field of medicine, fermentation technology, food technology and environmental technology are described. Guidelines for research with immobilized cells. Based on the scientific sessions a strategy of research and methods for characterization of immobilized cells, especially in view of applications are given.

The goal was to relate basic research to applications and to extract guidelines for characterization of immobilized cells in view of process design and application from the contributions. The manuscripts presented in these proceedings give an extensive and recent overview of the research and applications of immobilized-cell technology.

R.M. Buitelaar

Affiliations and Expertise

Agrotechnical Research Institute, ATO-DLO, Wageningen

C. Bucke

Affiliations and Expertise

University of Westminster, School of Biological and Health Sciences, London, U.K.

J. Tramper

Affiliations and Expertise

Wageningen Agricultural University, Department of Food Science and Technology, P.O.Box 8129, 6700 EV Wageningen, The Netherlands.

R.H. Wijffels

Affiliations and Expertise

Wageningen Agriculture University, Department of Food Science and Technology, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.

Immobilized Cells: Basics and Applications, 1st Edition

Due to space limitations, only a selected number of papers are listed.


Keynote lecture.

Why Immobilize? (C. Wandrey)

Basics 1: physics

Determination of biofilm diffusion coefficients using micro-electrodes (E.E. Beuling et al.). Fundamentals of dispersion in encapsulation technology (D. Poncelet, R.J. Neufeld). Adhesion of Lactococcus lactis diacetylactis to surfaces (S. Bourassa et al.). Screening of immobilization materials for anaerobic wastewater treatment (C.S. Hwu and S.-K. Tseng). Porous silicone rubber as an immobilization matrix for microbial and mammalian cells: natural immobilization of a mass transfer limited culture environment (A.J. Knights).Local mass transfer coefficients in bacterial biofilms using fluorescence recovery after photobleaching (FRAP) biofilms (J.D. Bryers, F. Drummond).

Basics 2: physiology, mass transfer and dynamic modelling

Immobilized-cell growth: diffusion limitation in expanding micro-colonies (R.H. Wijffels et al.). Competition and cooperation of microorganisms in a coimmobilized aerobic/anarobic mixed culture (G. John et al). Viability of immobilized cells: use of specific ATP levels and oxygen uptake rates (P. Gikas, A.G. Livingston). Nitrification activity of immobilized activated sludge evaluated by respiration rate (H. Nakamura et al.). Growth and eruption of gel-entrapped microcolonies (L.E. Hüsken et al.). Mass transfer limitations in a bioartificial pancreas (R. Willaert and G.V. Baron).

Immobilized-cell reactors

Scale up of immobilized-cell reactors (J.J. Heijnen). PEGASUS: innovative biological nitrogen removal process using entrapped nitrifiers (H. Emori et al.).


Industrial applications of immobilized biocatalysts in Japan (T. Shibatani). Immobilized cell technology in food processing (C.P. Champagne). Biological sulphate reduction with synthesis gas: microbiology and technology (R.T. Van Houten and G. Lettinga).


Guidelines for the characterization of immobilized cells (R.H. Wijffels et al.). Index of authors. Keyword index.

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