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Rational Design of Enzyme-Nanomaterials
1st Edition, Volume 571 - April 22, 2016
Editor: Challa Vijaya Kumar
Language: English
Hardback ISBN:9780128046807
9 7 8 - 0 - 1 2 - 8 0 4 6 8 0 - 7
eBook ISBN:9780128048337
9 7 8 - 0 - 1 2 - 8 0 4 8 3 3 - 7
Rational Design of Enzyme-Nanomaterials, the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by lead…Read more
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Rational Design of Enzyme-Nanomaterials, the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in rational design of enzyme-nanomaterials, and includes sections on such topics as conjugation of enzymes and dextran-aldehyde polymers, improved activity of enzymes bound to titanate nanosheet, nano-layered 'stable-on-the-table' biocatalysts and nanoparticle-based enzyme sensors.
Continues the legacy of this premier serial with quality chapters authored by leaders in the field
Covers research methods in rational design of enzyme-nanomaterials
Contains sections on such topics as conjugation of enzymes and dextran-aldehyde polymers, improved activity of enzymes bound to titanate nanosheet, nano-layered 'stable-on-the-table' biocatalysts, and nanoparticle-based enzyme sensors
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.
Preface
Acknowledgments
Chapter One: Preparation of Biocatalytic Microparticles by Interfacial Self-Assembly of Enzyme–Nanoparticle Conjugates Around a Cross-Linkable Core
Abstract
1 Theory
2 Equipment
3 Materials
4 Protocol
5 Step 1: Nanoparticle Synthesis
6 Step 2: Purification of Enzyme
7 Step 3: Preparation of the Aqueous Phase and Oil Phase
8 Step 4: Microparticle Assembly
9 Step 5: Microparticle Washing
10 Conclusions
Chapter Two: Monitoring Enzymatic Proteolysis Using Either Enzyme- or Substrate-Bioconjugated Quantum Dots
Abstract
1 Introduction
2 Quantification Assay for Observing Modified Kinetics with Enzyme–QD Conjugates
3 Enzyme Activity Sensors Based on Transient QD–Enzyme Interactions
4 Notes
Acknowledgments
Chapter Three: Intense PEGylation of Enzyme Surfaces: Relevant Stabilizing Effects
Abstract
1 Introduction
2 Theory
3 Protocols
4 Inactivation of Modified Enzyme Derivatives
5 Conclusions
Acknowledgments
Chapter Four: Immobilization of Lipases on Heterofunctional Octyl–Glyoxyl Agarose Supports: Improved Stability and Prevention of the Enzyme Desorption
Abstract
1 Theory
2 Equipment
3 Materials
4 Step 1. Preparation of the Support Octyl–Glyoxyl Agarose
5 Step 2. Immobilization of Lipases via Interfacial Activation on Octyl–Glyoxyl Agarose
6 Step 3. Covalent Immobilization of Adsorbed Lipases on Octyl–Glyoxyl Agarose
Acknowledgments
Chapter Five: Biomimetic/Bioinspired Design of Enzyme@capsule Nano/Microsystems
Abstract
1 Introduction
2 General Procedure of the Design and Construction of Enzyme@capsule Nano/Microsystems Through Biomimetic/Bioinspired Methods
3 Some Specific Examples
4 Concluding Remarks
Acknowledgment
Chapter Six: Synergistic Functions of Enzymes Bound to Semiconducting Layers
Abstract
1 Introduction
2 Fabrication of Enzyme-Intercalated Layered Oxides
3 Activity of Enzymes Bound to Titanate Layers
4 Photochemical Control of Enzymatic Activity of Oxidoreductases Bound to Layered Oxides
5 Biorecognition Using Doped Titanate Layers Modified with Biomolecules
6 Magnetic Application of Hybrids Composed of Enzymes and Doped Titanates
7 Conclusions
Acknowledgments
Chapter Seven: Bioconjugation of Antibodies and Enzyme Labels onto Magnetic Beads
Chapter Nine: Portable Enzyme-Paper Biosensors Based on Redox-Active CeO2 Nanoparticles
Abstract
1 Introduction
2 NPs-Based Enzyme Biosensors
3 CeO2 NPs for Enzyme Immobilization and Enzyme-Based Biosensors
4 Design of a CeO2-Based Colorimetric Enzyme Biosensor
5 Comments on the Method
Acknowledgments
Chapter Ten: Rational Design of Nanoparticle Platforms for “Cutting-the-Fat”: Covalent Immobilization of Lipase, Glycerol Kinase, and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles
Abstract
1 Introduction
2 Use of Rationally Designed Nanoscaffolds for Enzyme Binding
3 Use of Chitosan for Enhancing Nanoparticle Surface Chemistry
4 Experimental
Chapter Eleven: BioGraphene: Direct Exfoliation of Graphite in a Kitchen Blender for Enzymology Applications
Abstract
1 Introduction
2 Mechanism of Exfoliation
3 Tunability of the BioGraphene Characteristics
4 Protein Binding to Graphene and Some Biological Applications
5 Methods
6 Conclusions
Acknowledgments
Author Index
Subject Index
No. of pages: 294
Language: English
Edition: 1
Volume: 571
Published: April 22, 2016
Imprint: Academic Press
Hardback ISBN: 9780128046807
eBook ISBN: 9780128048337
CK
Challa Vijaya Kumar
Department of Chemistry, University of Connecticut, USA
Affiliations and expertise
Department of Chemistry, University of Connecticut, USA
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