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Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A
1st Edition, Volume 563 - October 5, 2015
Editors: Peter Z Qin, Kurt Warncke
Language: English
Hardback ISBN:9780128028346
9 7 8 - 0 - 1 2 - 8 0 2 8 3 4 - 6
eBook ISBN:9780128028469
9 7 8 - 0 - 1 2 - 8 0 2 8 4 6 - 9
Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Metho…Read more
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Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.
Timely contribution that describes a rapidly changing field
Leading researchers in the field
Broad coverage: Instrumentation, basic theory, data analysis, and applications
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.
Preface
Section I: EPR Methodology and Instrumentation
Chapter One: Rapid-Scan EPR of Nitroxide Spin Labels and Semiquinones
Abstract
1 Introduction
2 Rapid-Scan Method
3 Why Rapid Scan Improves S/N
4 Other Advantages of Rapid Scan
5 Hardware Requirements
6 Parameter Selection
7 Examples
8 Wider Scans
9 Future Applications
Acknowledgments
Chapter Two: Fourier Deconvolution Methods for Resolution Enhancement in Continuous-Wave EPR Spectroscopy
Abstract
1 Introduction
2 Historical Perspective
3 Overlapping Signals
4 Fourier Filtering
5 Overview of Method
6 Practical Examples
7 Limitations of the Method
8 Summary
Acknowledgment
Supplementary materials
Chapter Three: Multifrequency Pulsed EPR and the Characterization of Molecular Dynamics
Abstract
1 Introduction
2 Contributions to Electron Spin-Lattice Relaxation and Dependence of Relaxation on Motions in Fluid Solution
3 Measurement of Spin-Lattice Relaxation, T1
4 Examples of Motions Elucidated by Measuring T1 as a Function of Frequency
5 Conclusion
Acknowledgments
Chapter Four: Resolution and Characterization of Chemical Steps in Enzyme Catalytic Sequences by Using Low-Temperature and Time-Resolved, Full-Spectrum EPR Spectroscopy in Fluid Cryosolvent and Frozen Solution Systems
Abstract
1 Introduction
2 Low-Temperature Fluid Cryosolvent System
3 Low-Temperature Frozen Solution System
4 Analysis and Interpretations: General Cases and Guidelines
5 Conclusions
Acknowledgments
Chapter Five: 230/115 GHz Electron Paramagnetic Resonance/Double Electron–Electron Resonance Spectroscopy
Abstract
1 Introduction
2 HF EPR/DEER Spectrometer
3 Examples of HF EPR: Paramagnetic Impurities in Diamonds Studied by 230 GHz EPR/DEER Spectroscopy
4 Summary
Acknowledgments
Appendix
Section II: EPR Spectral Simulation and Software
Chapter Six: CW-EPR Spectral Simulations: Solid State
Chapter Fourteen: Development and Application of Spin Traps, Spin Probes, and Spin Labels
Abstract
1 Introduction
2 Spin Trapping
3 Spin Labeling
4 Conclusions
Acknowledgment
Chapter Fifteen: Site-Directed Spin Labeling of RNA by Postsynthetic Modification of 2′-Amino Groups
Abstract
1 Introduction
2 2′-Amino Spin-Labeling with Aliphatic Isocyanates and Aromatic Isothiocyanates
3 Summary and Conclusions
Acknowledgments
Chapter Sixteen: Gd3 + Spin Labeling for Measuring Distances in Biomacromolecules: Why and How?
Abstract
1 Introduction
2 Gd3 + Tags
3 Theoretical Background and the Effect of the High Spin
4 Relaxation
5 Experimental Set Up and Data Analysis
6 Multispin Effects
7 The SL Does Matter
8 In-Cell DEER
9 Outlook
Acknowledgments
Chapter Seventeen: Cu2 + as an ESR Probe of Protein Structure and Function
Abstract
1 Introduction
2 Cu2 + as a Probe to Measure Interspin Distances
3 Examples of Applications and Other Developments
Acknowledgment
Chapter Eighteen: Genetically Encoded Spin Labels for In Vitro and In-Cell EPR Studies of Native Proteins
Abstract
1 Introduction
2 Experimental Guidelines
Acknowledgments
Chapter Nineteen: Genetic Incorporation of the Unnatural Amino Acid p-Acetyl Phenylalanine into Proteins for Site-Directed Spin Labeling
Abstract
1 Introduction
2 Site-Specific Incorporation of pAcPhe
3 Spin Labeling of Proteins Containing Site-Specific pAcPhe
4 Inter-Nitroxide Distance Measurement in Doubly K1-Labeled PrP by DEER EPR
5 Concluding Remarks
Acknowledgments
Section V: Inter-Spin Label Distance Measurements: Analysis Considerations and Approaches
Chapter Twenty: A Straightforward Approach to the Analysis of Double Electron–Electron Resonance Data
Abstract
1 Introduction
2 Double Electron–Electron Resonance
3 Theory
4 Using DD
5 Error Analysis
6 The Background Signal
7 Global Analysis
8 Conclusions
Acknowledgments
Chapter Twenty-One: Computer Modeling of Spin Labels: NASNOX, PRONOX, and ALLNOX
Abstract
1 Introduction
2 NASNOX
3 PRONOX
4 ALLNOX
Chapter Twenty-Two: mtsslSuite: Probing Biomolecular Conformation by Spin-Labeling Studies
Abstract
1 Introduction
2 Planning the Project
3 Where to Put the Spin Label?
4 Selecting Optimal Labeling Sites
5 Which Spin Label Should Be Used?
6 Preparation of Spin-Labeled Samples and the PELDOR Experiment
7 Translating Distance Data into Structural Information
8 Caveats of In Silico Spin Labeling
9 Docking with mtsslDock
10 Trilateration with mtsslTrilaterate
11 Conclusion
Acknowledgments
Chapter Twenty-Three: Full Atom Simulations of Spin Label Conformations
Abstract
1 Introduction
2 Molecular Modeling
3 Simulation Strategy
4 Alternative Solution—Bifunctional Spin Labels
Author Index
Subject Index
No. of pages: 702
Language: English
Edition: 1
Volume: 563
Published: October 5, 2015
Imprint: Academic Press
Hardback ISBN: 9780128028346
eBook ISBN: 9780128028469
PQ
Peter Z Qin
Affiliations and expertise
Department of Chemistry, University of Southern California, USA
KW
Kurt Warncke
Affiliations and expertise
Department of Physics, Emory University, USA
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