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Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls
1st Edition - April 16, 2014
Author: John T. Corthell
Language: English
Paperback ISBN:9780128014615
9 7 8 - 0 - 1 2 - 8 0 1 4 6 1 - 5
eBook ISBN:9780128015278
9 7 8 - 0 - 1 2 - 8 0 1 5 2 7 - 8
Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scienti…Read more
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Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials.
Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding
Offers many details not present in other protocol books
Describes relevant controls for each technique and what those controls mean
Chapters include references (key articles, books, protocols) for additional study
Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules
Graduate and undergraduate students in biological and biomedical sciences, post-doctoral students and fellows, as well as all researchers seeking a practical resource on neurobiological methods.
Introduction
Chapter 1. General Notes
Aseptic Technique
Aliquoting
Chapter 2. DNA and RNA Extraction Protocols
Phenol:Chloroform (TRIzol™) Extraction Notes
Phenol:Chloroform Extraction Protocol
RNA Extraction
DNA Extraction
Protein Extraction
Kit Extraction of RNA Notes
Solution Recipes
Chapter 3. Agarose Gel Electrophoresis
Electrophoresis Notes
Agarose Gel Electrophoresis Protocol
Solution Recipes
Chapter 4. Reverse Transcription (RT) and Polymerase Chain Reaction (PCR)
Reverse Transcription Notes
Polymerase Chain Reaction (PCR) Notes
End-Point PCR
Primer Design
Reverse-Transcription PCR (RT-PCR)
Quantitative PCR (Real-Time PCR or qPCR)
Quantitation Methods
Running a Plate and Data Analysis
PCR Controls
PCR Troubleshooting
Chapter 5. Antibodies and Titrations
Chapter 6. Protein Extraction
Protein Extraction Notes
Total Cytosolic/Membrane Protein Extraction Protocol
Subcellular Fractionation Protocol (Adapted from Two Papers)8,9
ISH Probe Design for Oligonucleotide and PCR Probes
ISH Protocol (Developed from Two Sources30,31)
ISH Controls23,33
Solution Recipes
References
No. of pages: 124
Language: English
Edition: 1
Published: April 16, 2014
Imprint: Academic Press
Paperback ISBN: 9780128014615
eBook ISBN: 9780128015278
JC
John T. Corthell
Dr. Corthell received a PhD in Neuroscience from Florida State University, where he studied circadian rhythms using whole-cell patch-clamp electrophysiology, as well as numerous molecular biology techniques, in the olfactory system. He has more than 10 years of experience in laboratory work and techniques in the biological and chemical sciences, and more than 5 years teaching. The author has cultivated these techniques over the years through reading, asking questions, and trial and error. Each of these techniques has been tested, performed, and in some cases optimized by the author himself. He enjoys reading, writing, learning, children, and big dogs. When not working, he longs to go SCUBA diving.
Affiliations and expertise
University of Michigan, Ann Arbor, MI, USA
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