Key Features
@introbul:Key Features
@bul:* Provides a practical laboratory guide for studying cell nuclei
* Includes comprehensive and easy-to-follow protocols
Description
This volume is a comprehensive guide to the methodologies used in the study of structural domains of cell nuclei. The text covers chromatin, the karyoskeleton, the soluble domain, and the nucleolus. It details methods that are used to isolate components from these domains and techniques used to assemble and disassemble nuclear elements. There is also coverage of three-dimensional mapping and localization of nuclear processes.
Readership
Graduate students, technicians, postdoctoral, and experienced researchers. Cell, developmental, and molecular biologists, geneticists, human genome researchers, and others studying nuclear structure and organization.
Nuclear Structure and Function, 1st Edition
M. Berrios, Introduction.
Cell Fractionation Preparation and Characterization of Probes:M. Berrios, Isolation and Characterization of Nuclear Pore Complex-Lamina Fractions from Vertebrates.
P.A. Fisher,Preparation and Characterization of Karyoskeletal Protein-Enriched Fractions from
Drosophila melanogaster.J.P. Aris, Isolation of Nuclei and Nucleoli from Yeast
Saccharomyces cerevisiae.Structural Analysis of the Interphase Nucleus:M.R. Paddy, Qualitative and Quantitative Approaches to the Spatial Localization of Nuclear Proteins with the Fluorescence Light Microscope.
U. Kubitscheck and R. Peters, Localization of Single Nuclear Pore Complexes by Confocal Laser Scanning Microscopy and Analysis of their Distribution.
A.S. Belmont, Nuclear Ultrastructure Transmission Electron Microscopy and Image Analysis.
T.D. Allen, S.A. Rutherford, G.R. Bennion, M.W. Goldberg, and E. Kiseleva, Three-Dimensional Surface Structure Analysis of the Nucleus.
J. Hainfeld and J. Wall, Scanning Transmission Electron Microscopy of Nucleic Acid-Protein Complexes.
Chromatin and Sub-Nuclear Structures:C.L. Woodcock and R.A. Horowitz, Chromatin and Nucleosomal Assemblies.
A.F. Dernburg and J.W. Sedat, Mapping the Architecture of Interphase Nucleus.
A.J. Solari, Structural Analysis of Meiotic Chromosomes and Synaptonemal Complexes in Higher Vertebrates.
J. Engebrecht, F. Klein, and J. Loidl, Genetic and Morphological Approaches for the Analysis of Meiotic Chromosomes in Yeast.
M. Grote and R. Peters, Mapping Proteins to Nuclear Pore Complexes by Immunogold Electron Microscopy.
R.L. Ochs, Methods to Study the Structure and Function of the Nucleolus.
T. Kohwi-Shigematsu, I. DeBelle, L.A. Dickinson, S. Galande, and Y. Kowi, Identification of Base-Unpairing Region (BUR)-Binding Proteins and Characterization of their
In Vivo Binding Sequences.
Nuclear Assembly/Disassembly Cell-Free Systems:B. Burke, Mammalian Systems to Study Nuclear Assembly.
M.J. Lohka and D.A. Warren, Analysis of Nuclear Envelope Assembly Using Extracts of
Xenopus Eggs.
P.A. Fisher and M. Berrios, Methods for Studying Nuclear Assembly and Disassembly Using
Drosophila Cell-Free Systems.
P. Collas and D.L. Poccia, Methods for Studying
in Vitro Assembly of Male Pronuclei Using Oocyte Extracts from Marine Invertebrates Sea Urchins and Surf Clams.
Localization of Nuclear Processes:R. Van Driel, E.M.M. Manders, L. de Jong, and J.A. Aten, Mapping Sites of DNA Replication In Situ.
Y. Osheim and A.L. Beyer, Analysis of RNA Transcription and Processing by Transmission Electron Microscopy.
G.H. Leno, Cell-Free Systems to Study Chromatin Remodeling.
C. Dingwall and I. Palacios, Reconstruction of Nuclear Protein and snRNP Import
In Vitro.
D.S. Goldfarb, Measuring Nuclear Transport Efficiency in
Saccharomyces cerevisiae Using a Nuclear Localization Signal-Tagged Green Fluorescent Protein.
M.P. Terns and S. Goldfarb, The Nuclear Transport of Microinjected RNAs in
Xenopus Oocytes.
R. Benavente and G. Krohne,
In Vivo Systems to Study the Dynamics of Nuclear Lamins.
Y. Lazebnik, Apoptosis a Model System to Study the Structural Collapse of the Nucleus.
Subject Index.
