Key Features
@introbul:Key Features
@bul:* Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes
* Fluorescent dyes
* Luminescent proteins
* Includes techniques for perturbing intracellular Ca2+
* Covers detailed methodology plus problems and pitfalls of each technique
* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
* Color plates illustrate techniques such as
* Confocal ratio-imaging
* Use of aequorin
Description
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.
Readership
Researchers, graduate students, and technicians in cell, developmental, and molecular biology, neuroscience, physiology, biochemistry, biophysics, and optical sciences.
A Practical Guide to the Study of Calcium in Living Cells, 1st Edition
Ca2+ Buffers and [Ca2+]I Perturbation Techniques:D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca
2+ Buffers.
R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca
2+.
D. Thomas and M.R. Hanley, Pharmacological Tools for Perturbing Intracellular Calcium Storage.
Microelectrode Techniques for Measuring [Ca2+]I and Ca2+ Fluxes:S. Baudet, L. Hove-Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes.
P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca
2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca
2+ Influx and Efflux.
C.A. Leech and G.G. Holz, IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels.
Fluorescence Techniques for Imaging [Ca2+]I:J.P.Y. Kao, Practical Aspects of Measuring [Ca
2+] with Fluorescent Indicators.
S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH.
T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques.
P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca
2+ in Cells.
S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in
Xenopus Oocytes.
Use of Aequorin for [Ca2+]I Imaging:K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca
2+]I Changes Using a Photomultiplier Tube.
A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca
2+]I with Aequorin Using a Photon Imaging Detector.
R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles.
Index.
Quotes and reviews
@qu:"This multi-authored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca2+]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced... The book provides a pragmatic approach for the estimation of cytosolic [Ca2+]i and also provides a means for studying the spatial and temporal resolution of [Ca2+]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research.' It is a very well-written monograph and includes useful illustrations and helpful, practical points."
@source:--RAMESH BHALLA, University of Iowa, Iowa City
@qu:"The book achieves the stated goal in the preface, this material will be very useful to the investigator who wants to apply any of these methods to his or her research. It is a very well-written monograph and includes useful illustrations and helpful, practical points."
@source:--MOLECULAR & CELLULAR NEUROSCIENCES
