Key Features
@introbul:Key Features
@bul:* Contains both theoretical and practical issues in 30 chapters contributed by the world authorities on Tetrahymena
* Indispensible for both the novice and the experienced researcher
* Overviews the history, cell biology, and genetics of the organism
* Describes essential protocols on the growth of cells, genetic techniques, and how to look at the cells with the microscope
* Illustrates how the methods can be applied to solve various cell biological problems
* Reviews recently developed strategies for altering gene expression
Description
Tetrahymena thermophila is emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations with T. thermophila have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for using Tetrahymena in the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning to Tetrahymena as the experimental system of choice.
Readership
Cell biologists, developmental biologists, geneticists, and molecular biologists.
Tetrahymena Thermophila, 1st Edition
Overview:D.L. Nanney and E.M. Simon, Laboratory and Evolutionary History of
Tetrahymena thermophila.
J. Frankel, Cell Biology of
Tetrahymena thermophila.
K.M. Karrer,Tetrahymena Genetics: Two Nuclei are Better Than One.
Methods:E. Orias, E.P. Hamilton, and J.D. Orias,
Tetrahymena as a Laboratory Organism: Useful Strains, Cell Culture, and Cell Line Maintenance.
P.J. Bruns, H.R. Smith, and D. Cassidy-Hanley, Long-term Storage.
E.P. Hamilton and E. Orias, Genetic Crosses: Setting up Crosses, Testing Progeny, and Isolating Phenotypic Assortants.
P.J. Bruns and D. Cassidy-Hanley, Methods for Genetic Analysis.
S.L. Allen, Isolation of Micronuclear and Macronuclear DNA.
E. Orias and E.P. Hamilton, Genetically Sorting a Collection of
Tetrahymena Mutants.
E.P. Hamilton and E. Orias, Genetically Mapping New Mutants and Cloned Genes.
D.G. Pennock, Selection of Motility Mutants.
K.R. Stuart and E.S. Cole, Nuclear and Cytoskeletal Fluorescence Microscopy Techniques.
E.S. Cole and K.R. Stuart, Nuclear and Cortical Histology for Brightfield Microscopy.
W. Dentler, Fixation of
Tetrahymena Cells for Electron Microscopy.
R.H. Gavin, J.G. Hoey, and J.A. Garcés, Immunoelectron Microscopy of
Tetrahymena.
Cell Biology:A.P. Turkewitz, N.D. Chilcoat, A. Haddad, and J.W. Verbsky, Regulated Protein Secretion in
Tetrahymena thermophila.
T.M. Hennessey and H.G. Kuruvilla, Electrophysiology of
Tetrahymena.
E.A. Wiley, C.A. Mizzen, and C.D. Allis, Isolation and Characterization of
in Vivo Modified Histones and an Activity Gel Assay for Identification of Histone Acetyltransferase.
C. Autexier,
Tetrahymena Telomerase Activity, Purification, and Reconstruction.
E. Blackburn, D. Gilley, T. Ware, A. Bhattacharyya, K. Kirk, and H. Wang, Studying the Telomerase RNA in
Tetrahymena.
T.M. Gibson and D.J. Asai, Isolation and Characterization of 22S Outer Arm Dynein from
Tetrahymena Cilia.
N.E. Williams Preparation of Cytoskeletal Fractions from Tetrahymena thermophila.
N.E. Williams, Immunoprecipitation Procedures.
O. Numato, K. Hanyu, T. Takeda, and Y. Watanabe, Tetrahymena Calcium-Binding Proteins, TCBP-25 and TCBP-23.
Manipulating Genes:D.L. Chalker, J.G. Ward, C. Randolph, and M.-C. Yao, Microinjection of
Tetrahymena thermophila.
J. Gaertig and G. Kapler, Transient and Stable DNA Transformation of
Tetrahymena thermophila by Electroporation.
P.J. Bruns and D. Cassidy-Hanley, Biolistic Transformation of Macro- and Micronuclei.
B. Hai, J. Gaertig, an M.A. Gorovsky, Knockout Heterokaryons Enable Facile Mutagenic Analysis of Essential Genes in
Tetrahymena.
Q. Fan, R. Sweeney, and M.-C. Yao, Creation and Use of Antisense Ribosomes in
Tetrahymena thermophila.
L. Yu and M.A. Gorovsky, Protein Tagging in
Tetrahymena.
Appendix I: Genetic Nomenclature Rules for
Tetrahymena thermophila.
Appendix II: Codon Usage in
Tetrahymena thermophila.
Chapter References.
Index.
Volumes in Series.
