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Antibody Techniques
1st Edition - September 13, 1994
Editors: Vedpal S. Malik, Erik P. Lillehoj
Language: English
eBook ISBN:9781483285139
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The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since…Read more
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The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to anyone doing biological or biochemical research. This practical guide to immunotechniques is especially designed to be easily understood by people with little practical experience using antibodies. It clearly presents detailed, easy-to-follow, step-by-step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage.
Researchers and technicians in molecular biology, cell biology, developmental biology, virology, microbiology, pharmacology, and biotechnology.
I.M. Zitron, Antibody Molecules and the Immune Response.D.P. Snider, Adjuvant-Free Polyclonal Antibody Response Manipulated by Antibody-Mediated Antigen Targeting.C. Barrett, Hybridomas and Monoclonal Antibodies.J.W. Larrick and R.F. Balint, PCR Amplification and Antibody Genes.P.C. Allen, Laboratory Animal Care and Use.A.C. Allison and N.E. Byars, Adjuvants.C. Glad and I. Nilsson, Large-Scale Production of Monoclonal Antibodies.D.P. Boyle and D.W. Metzger, Antibody-Binding Bacterial Proteins as Immunoreagents.B.-K. Choe, Conjugating Antibodies to Ligands, Enzymes, and Metallic Particles.E. Lauritzen, H. Flyge, and A. Holm, Dot Immunobinding (DIB), Enzyme Linked Immunosorbent Assay (ELISA) and Radioimmunoassay (RIA) for Detection of Peptide Antigens and Specific Antibodies.B.-K. Choe and Y. Cho, Immunostaining Cells and Tissues.E.P. Lillehoj, Protein Immunoblotting.H.S. Lillehoj and A.G. Martin, Flow Cytometry and Fluoresence-Activated Cell Sorting.W.J. Patrie, Screening Expression Libraries with Antibodies.J.P. Tharakan, Immunoaffinity Purification.
No. of pages: 353
Language: English
Edition: 1
Published: September 13, 1994
Imprint: Academic Press
eBook ISBN: 9781483285139
VM
Vedpal S. Malik
Affiliations and expertise
U.S. Department of Agriculture, APHIS, Hyattsville, Maryland, U.S.A.
EL
Erik P. Lillehoj
Affiliations and expertise
The George Washington University Medical Center, Washington, D.C., U.S.A.