Manual of Techniques in Invertebrate Pathology

Manual of Techniques in Invertebrate Pathology, 2nd Edition

Manual of Techniques in Invertebrate Pathology, 2nd Edition,Lawrence Lacey,ISBN9780123868992

L Lacey   

Academic Press




276 X 216

Now including pathogens of additional invertebrates, the 2nd edition provides essential coverage of step-by-step protocols for the isolation, identification, propagation, bioassay and preservation of entomopathogens.

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Key Features

  • Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens
  • New edition fully updated to address changes in the taxonomy of the vast majority of taxa
  • Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques
  • Provides extensive supplemental literature and recipes for media, fixatives and stains


Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful.


Insect pathologists; biological control and integrated pest management specialists; entomologists; marine biologists, microbiologists; nematologists; protozoologists; virologists; mycologists and biologists involved in basic and applied research on invertebrate pathogens

Lawrence Lacey

Affiliations and Expertise

IP Consulting International, Yakima, WA, USA

Manual of Techniques in Invertebrate Pathology, 2nd Edition

Manual of Techniques in Invertebrate Pathology, 2nd edition

Lawrence A. Lacey, editor

1. Initial Handling and Diagnosis of Diseased Invertebrates

Lawrence A. Lacey and Leellen Solter

2. Basic Techniques in Insect Virology

Karolin E. Eberle, Jörg T. Wennmann, Regina G. Kleespiesand Johannes A. Jehle

3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli.

Tanja W. Fisher and Steven F. Garczynski

4. Bioassay of Bacterial Entomopathogens Against Insect Larvae

Maureen O’Callaghan, Travis R. Glare and Lawrence A. Lacey

5. Bacteria for use Against Soil-Inhabitating Insects

Albrecht M. Koppenhöfer, Trevor A. Jackson, and Michael G. Klein

6. Identification of Entomopathogenic Fungi

Richard A. Humber

7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales

G. Douglas Inglis, Juerg Enkerli, and Mark S. Goettel

8. Mass Production of Entomopathogenic Hypocreales

Stefan T. Jaronski and Mark A. Jackson

9. Methods for the Study of Entomophthorales

Ann E. Hajek, Bernard Papierok, and Jørgen Eilenberg

10. Preservation of Entomopathogenic Fungal Cultures

Richard A. Humber

11. Research Methods for Entomopathogenic Microsporidia and Other Protists

Leellen Solter, James Becnel, and Jiri Vávra

12. Nematode Parasites, Pathogens and Associates of Insects and Invertebrates of Economic Importance

S. Patricia Stock and Heidi Goodrich-Blair

13. Pathogens and Parasites of Terrestrial Molluscs

Michael J. Wilson

14. Testing the Pathogenecity and Infectivity of Entomopathogens to Mammals

Joel P. Siegel

15. Complementary Techniques: Preparations of Entomopathogens and Diseased Specimens for More Detailed Study Using Microscopy

James J. Becnel

Quotes and reviews

listing in Society of Invertebrate Pathology Newsletter


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