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Laboratory Methods in Enzymology: RNA
 
 

Laboratory Methods in Enzymology: RNA, 1st Edition

 
Laboratory Methods in Enzymology: RNA, 1st Edition,Jon Lorsch,ISBN9780124200371
 
 
 

Methods in Enzymology

  

Academic Press

9780124200371

472

229 X 152

In this volume we have brought together a number of core protocols concentrating on RNA, carefully written and edited by experts.

Print Book

Hardcover

In Stock

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USD 199.00
 
 

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on RNA

Description

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Jon Lorsch

Affiliations and Expertise

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA

View additional works by Jon Lorsch

Laboratory Methods in Enzymology: RNA, 1st Edition

Computational Prediction of RNA Secondary Structure

Reverse-transcription PCR (RT-PCR)

Northern blotting

Explanatory Chapter: Nuclease Protection Assays

In Vitro Transcription from Plasmid or PCR-amplified DNA

Measurement of In Vivo RNA Synthesis Rates

Method for measuring mRNA decay rate in Saccharomyces cerevisiae

Analysis of polysomes from bacteria

Polysome Profile Analysis – Yeast

Polysome analysis of mammalian cells

Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae

Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates

Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3'; ends, and poly(A) tails of RNA

RNA Radiolabeling

Fluorescently Labeling Synthetic RNAs

Analysis of RNA by analytical polyacrylamide gel electrophoresis

RNA purification by preparative polyacrylamide gel electrophoresis

Explanatory Chapter: Nucleic Acid Concentration Determination

RNA purification – precipitation methods

Reverse Transcriptase Dideoxy Sequencing of RNA

RNA Structure Experimental Analysis – Chemical Modification

Structural Analysis of RNA Backbone Using In-Line Probing

 
 

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