Correlative Light and Electron MIcroscopy

Correlative Light and Electron MIcroscopy, 1st Edition

Correlative Light and Electron MIcroscopy, 1st Edition,Thomas Mueller-Reichert,Paul Verkade,ISBN9780124160262

Methods in Cell Biology

Mueller-Reichert   &   Verkade   

Academic Press




235 X 191

This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

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Key Features

  • Covers many areas of CLEM by the best international scientists in the field
  • Includes a brief history and overview on CLEM methods
  • Description

    The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.


    Researchers and students in cell, molecular and developmental biology

    Thomas Mueller-Reichert

    Dr. Thomas Müller-Reichert is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in meiosis, mitosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in the early embryo of the nematode Caenorhabditis elegans and in tissue culture cells. He got his PhD degree from the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards to the EMBL in Heidelberg (Germany) for a post-doc with Dr. Tony Hyman. He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA) and set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is head of the Core Facility Cellular Imaging (CFCI) of the Medical Faculty Carl Gustav Carus of the TU Dresden (Germany). Together with Dr. Paul Verkade he has developed a rapid transfer system for high-pressure freezing used for Correlative Light and Electron Microcopy. He has organized a number microscopy conferences and taught in several (CL)EM courses. He edited an MCB volume on the Electron Microscopy of Model Systems.

    Affiliations and Expertise

    Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, Dresden, Germany

    View additional works by Thomas Mueller-Reichert

    Paul Verkade

    Dr. Paul Verkade’s research focuses on the sorting mechanisms in intracellular transport pathways. His main tools are microscopy techniques, with an emphasis on electron microscopy (EM) in which field he has published over 50 papers. He has studied and got his PhD degree at the University of Utrecht, the Netherlands. After his post-doc time at the EMBL, Heidelberg, Germany in the group of Kai Simons and setting up a new EM lab at the Max Planck Institute for Molecular Cell Biology in Dresden, Germany he moved to the University of Bristol, UK in 2006. Here he set up a new EM unit as part of the Wolfson Bioimaging Facility, a fully integrated light and electron microscopy centre. To support his transport studies, part of his research is to develop techniques and tools for the use of Correlative Light Electron Microscopy (CLEM). Amongst other things he has developed the Rapid Transfer System for the EMPACT2 high-pressure freezer together with Leica Microsystems. This allows for the combination of time-resolved CLEM with optimal preservation of ultrastructure for EM. Dr. Verkade is chair of the Electron Microscopy section of the Royal Microscopical Society (RMS) and of the Cryo Microscopy Group, affiliated to the RMS. He has organised and taught on a large number of courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy, and immuno EM. He is also the principle organiser of the EMBO practical course on CLEM.

    Affiliations and Expertise

    Wolfson Bioimaging Facility, Schools of Biochemistry and Physiology & Pharmacology, University of Bristol, Bristol, UK

    Correlative Light and Electron MIcroscopy, 1st Edition

    1. Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
    2. K. Kobayashi, D. Cheng, M. Huynh, K.R. Ratinac, P. Thordarson & F. Braet

    3. Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy.
    4. Roman S. Polishchuk, Elena V. Polishchuk, and Alberto Luini

    5. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
    6. Toshihiro Takizawa and John M. Robinson

    7. Correlative Light and Electron Microscopy in Parasite Research
    8. Céline Loussert, Claire-Lise Forestier and Bruno M. Humbel

    9. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
    10. Gunar Fabig, Susanne Kretschmar, Susanne Weiche, Dominic Eberle, Marius Ader, and Thomas Kurth

    11. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
    12. Katia Cortese, Giuseppe Vicidomini, MariaCristina Gagliani1, Patrizia Boccacci, Alberto Diaspro, and Carlo Tacchetti

    13. Correlative Light and Electron Microscopy of GFP
    14. Markus Grabenbauer

    15. Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
    16. Mark H. Ellisman, Thomas J. Deerinck, Xiaokun Shu and Gina E. Sosinsky

    17. Correlated Light Microscopy and Electron Microscopy (CLEM): Search… and Find!
    18. Klaas A. Sjollema, Ulrike Schnell, Jeroen Kuipers, Ruby Kalicharan & Ben N. G. Giepmans

    19. Capturing Endocytic Segregation Events with HPF-CLEM
    20. Edward Brown, Jan van Weering, Thom Sharp, Judith Mantell and Paul Verkade

    21. Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
    22. Irina Kolotuev, Daniel J Bumbarger, Michel Labouesse and Yannick Schwab

    23. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo
    24. Ina Woog, Silke White, Mandy Büchner, Martin Srayko and Thomas Müller-Reichert

    25. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
    26. Wanda Kukulski, Martin Schorb, Sonja Welsch, Andrea Picco, Marko Kaksonen and John A. G. Briggs

    27. Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
    28. Alexander Rigort, Elizabeth Villa, Felix J. B. Bäuerlein and Jürgen M. Plitzko

    29. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
    30. Shigeki Watanabe and Erik M. Jorgensen

    31. Atmospheric Scanning Electron Microscope for Correlative Microscopy
    32. Ian E.G. Morrison, Clare L. Dennison, Hidetoshi Nishiyama, Mitsuo Suga, Chikara Sato, Andrew Yarwood and Peter J. O'Toole

    33. Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
    34. Miriam Lucas, Maja Günthert, Philippe Gasser, Falk Lucas and Roger Wepf

    35. Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms

    Andrew J. Bushby, Giovanni Mariggi, Hannah E.J. Armer and Lucy M. Collinson

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