- Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems
- Includes detailed drawings that complement both introductions and experiments
- Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
- Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology
- Fully updated literature
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.
The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.
Faculty, graduate students, and undergraduates interested in plant cell and tissue culture.
Plant Tissue Culture, 3rd Edition
Plant Tissue Culture: Techniques and Experiments
Roberta H. Smith
Table of Contents
Scheduling and Interrelationships of Exercises
1. History of Plant Cell Culture
Trevor A. Thorpe
2. Setup of a Tissue Culture Laboratory
3. Media Components and Preparation
4. Explant Preparation
6. Callus Induction
7. Regeneration and Morphogenesis
8. Woody Shrubs and Trees
Brent H. McCown
9. Haploid Plants from Anther Culture
10. Embryo Rescue
11. Meristem Culture for Virus-Free Plants
12. In Vitro Propagation for Commercial Production of Ornamentals
13. Protoplast Isolation and Fusion
Jungeun Kim Park, Sunghun Park, & James E. Craven
14. Agrobacterium-Mediated Transformation of Plants
Jungeun Kim Park, Sunghun Park, Qingyu Wu, & Stuart Sprague
Appendix I: Useful Measurements
Appendix II: Review of Solution Preparation
Appendix III: List of Suppliers
Appendix IV: Common Plant Tissue Culture Terms
Quotes and reviews
"The strength of this book lies in its descriptions of essential concepts, introduction to a wide range of major applications, and simple protocols to provide empirical demonstrations. ...this book would be a good teaching resource reference for instructors wishing to design their own laboratory demonstations."
@source:--Phil Bregitzer, Germplasm Research Facility, in CROP SCIENCE SOCIETY OF AMERICA (June 2001)
@qu:"...presents a structured programme using plant material that is available all year round. ...I was impressed with the gradual introduction of complex techniques and ideas. ...the book is well written...a useful contribution to teaching in plant tissue culture."
@source:-Ronald A. de Fossard in TRENDS IN PLANT SCIENCE (February 2001)
@from:Praise for the First Edition
@qu:"This is an excellent laboratory manual. The student can learn many plant tissue culture techniques commonly used today."
@qu:"This book is an excellent overview of important tissue culture techniques. It is highly recommended for laboratory courses in tissue culture and would be useful in research laboratories where tissue culture techniques are required. Practical aspects of tissue culture are explained in a clear and concise way. This is essential for the novice and useful for experienced individuals planning a new course or laboratory. The manual is commended for providing information required for successful tissue culture at the fingertips of students."
@source:--JOURNAL OF NATURAL RESOUCES AND LIFE SCIENCES EDUCATION (1993)