- Provides information on how technology works
- Discusses how and why to prepare samples
- Instructs on how to analyze data
- Covers basic techniques and history behind the kit culture
Next Generation Sequencing demystifies the process of sequencing with a hands-on approach. Offering a theoretical and practical discussion of the how and why behind the kit culture, this publication instructs readers on the science behind the technology and the analysis and application of data. The primer includes a needed pros-and-cons list, protocols, and a discussion of implementing applications.
Filling the gap in next generation sequencing literature, Next Generation Sequencing: A Primer provides needed instruction for early researchers, students and researchers outside of their expertise across the biological and biomedical sciences.
Next Generation Sequencing, 1st Edition
Current next generation technologies, clonal approaches, single molecule approaches
Rationale: NGS enables new biological questions
Growth of cells for NGS (using yeast and cultured mammalian cells)
Nucleic acid extraction: DNA and RNA
PCR, gel electrophoresis and quantitative PCR
Overview of diverse types of NGS sequencing libraries.
Preparation of genomic DNA and RNA-seq libraries
Sequencer Operation: overview of diverse platforms
Sequencing on the MiSeq
Data extraction and analysis I: quality filters, trimming, demultiplexing and mapping
Data analysis II: genome viariants- SNPs, CNVs and rearrangements
Data analysis III: RNA seq analysis- gene expression, non-coding sequences, splicing
Data analysis IV: Data visualization and data sharing
Summary and report preparation
Applications of NGS
Perspectives: NEXT next generation technologies