»
Fluorescence Fluctuation Spectroscopy (FFS) Part B
 
 

Fluorescence Fluctuation Spectroscopy (FFS) Part B, 1st Edition

 
Fluorescence Fluctuation Spectroscopy (FFS) Part B, 1st Edition,Sergey Tetin,ISBN9780124055391
 
 
Up to
25%
off
 

Methods in Enzymology

S Tetin   

Academic Press

9780124055391

9780124058576

376

229 X 152

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells

Print Book + eBook

USD 231.60
USD 386.00

Buy both together and save 40%

Print Book

Hardcover

In Stock

Estimated Delivery Time
USD 149.25
USD 199.00

eBook
eBook Overview

VST (VitalSource Bookshelf) format

DRM-free included formats : EPUB, Mobi (for Kindle), PDF

USD 140.25
USD 187.00
Add to Cart
 
 

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers fluorescence fluctuation spectroscopy
  • Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells

Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Information about this author is currently not available.

Fluorescence Fluctuation Spectroscopy (FFS) Part B, 1st Edition

Contributors

Preface

Methods in Enzymology

Chapter One. FCS in STED Microscopy

1 Introduction

2 STED-FCS of Lipid Membrane Dynamics

3 Lipid Membrane Dynamics

4 Conclusions

References

Further Reading

Chapter Two. Analyzing Förster Resonance Energy Transfer with Fluctuation Algorithms

1 Introduction

2 FRET and FCS

3 Filtered FCS

4 Applications

5 Discussion

References

Chapter Three. Fluorescence Fluctuation Spectroscopy Approaches to the Study of Receptors in Live Cells

1 Introduction

2 Selected FFS Studies

3 Choice of Fluorophores: General Considerations

4 Cells: General Considerations

5 Summary

References

Chapter Four. Studying the Protein Corona on Nanoparticles by FCS

1 Introduction

2 Sample Preparation

3 Experimental Procedures

4 Data Analysis

5 Protein Corona Formation Measured by FCS

6 Conclusions

References

Chapter Five. Studying Antibody–Antigen Interactions with Fluorescence Fluctuation Spectroscopy

1 Introduction

2 Binding Model and Experimental Considerations

3 Studying Antibodies with FFS

4 Instrumentation

References

Chapter Six. Fluorescence Fluctuation Approaches to the Study of Adhesion and Signaling

1 Introduction

2 A Fluorescence Fluctuation Toolbox

3 Experimental Implementation

4 Applications

5 Conclusion

References

Chapter Seven. Interactions in Gene Expression Networks Studied by Two-Photon Fluorescence Fluctuation Spectroscopy

1 Introduction

2 In Vitro Interactions Between Proteins and Nucleic Acids Using Fluctuation Approaches

3 FFM in Live Bacterial Cells

4 Conclusions and Perspectives

References

Chapter Eight. Studying Ion Exchange in Solution and at Biological Membranes by FCS

1 Introduction

2 Ion Exchange Monitoring by FCS—Basic Approach

3 Monitoring of Local Ion Concentrations and Exchange in Solution

4 Monitoring of Proton Exchange at Biological Membranes by FCS

5 Approach for Ion Exchange Monitoring Incorporating Dual Color Fluorescence Cross Correlation Spectroscopy (FCCS)

6 Conclusions

References

Chapter Nine. Fluctuation Analysis of Activity Biosensor Images for the Study of Information Flow in Signaling Pathways

1 Introduction

2 Activity Biosensors

3 Extracting Activity Fluctuations in a Cell Shape Invariant Space

4 Correlation Analysis of Activity Fluctuations for Pathway Reconstruction

5 Outlook

References

Chapter Ten. Probing the Plasma Membrane Organization in Living Cells by Spot Variation Fluorescence Correlation Spectroscopy

Abbreviations

1 Introduction

2 Optical Setups for Sizing the Excitation Volume

3 General Considerations for svFCS Acquisition

4 Measurements on Living Cells

5 svFCS Data Analysis and Curve Fitting

6 The Nature of the Molecular Constraints on Lateral Diffusion

7 Summary and Future Outlook

References

Author Index

Subject Index

 
 
Free Shipping
Shop with Confidence

Free Shipping around the world
▪ Broad range of products
▪ 30 days return policy
FAQ

Contact Us