Volume 524: Cilia, Part A, 1st Edition

Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.

Cilia, Part A, 1st Edition

1. Electron microscopy of IFT in cilia and flagella
   Stefan Geimer
2. Biochemical analysis of ciliary transport

Peter Jackson

3. Visualization of IFT in Chlamydomonas flagella

Karl F. Lechtreck

4. Visualization of kidney cilia-mediated calcium signaling

Jing Zhou

5. Methods for analyzing nodal cilia and flow

Shigenori Nonaka

6. Methods for building synthetic cilia-like motor-microtubule arrays

Zvonimir Dogic

7. Isolation of IFT particle proteins from Chlamydomonas

Hongmin Qin

8. Radial Spoke isolation and assays

Pinfen Yang

9. Biomechanical measurements of kinocilium

Wally Grant

10. Methods for analysis of Calcium/calmodulin signaling in flagella

Elizabeth Smith

11. Ubiquitination of Flagellar Proteins

Kaiyao Huang Sr.

12. Visualizing IFT in C. elegans cilia

Jonathan M. Scholey

13. Proteomic analysis of mammalian primary cilia

Hiroaki Ishikawa

14. TSC and TOR signaling in cilia

Zhaoxia Sun

15. Bioinformatic approaches to cilia

Peter Swoboda

16. Methods for testing cilia function in cell migration

Søren Tvorup Christensen

17. Signaling and waveform in Drosophila sperm flagella

Xiangyi Lu

18. Analysis of import into primary cilia

Kristen Verhey

19. Patch-Clamp Recording from Mouse and Human Spermatozoa

Yuriy Kirichok

20. Optical trap measurements of flagellar membrane motility
    William Guilford