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Metabolomics Coming of Age with its Technological Diversity
 
 

Metabolomics Coming of Age with its Technological Diversity, 1st Edition

 
Metabolomics Coming of Age with its Technological Diversity, 1st Edition,Dominique Rolin,ISBN9780123984784
 
 
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D Rolin   

Academic Press

9780123984784

712

This is the 67th volume of the Advances in Botanical Research, and features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology.

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Key Features

  • Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences
  • Features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology
  • Volume features reviews on metabolomics coming of age with its technological diversity

Description

Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 67th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume features reviews on metabolomics coming of age with its technological diversity.

Readership

Postgraduates and researchers in plant sciences, including botany, plant biochemistry, plant physiology, plant pathology, virology, entomology, and molecular biology

Information about this author is currently not available.

Metabolomics Coming of Age with its Technological Diversity, 1st Edition

Series Page

Contributors

Chapter One. High-Resolution 1H-NMR Spectroscopy and Beyond to Explore Plant Metabolome

1 Introduction

2 NMR Signals: Measurable Properties

3 Metabolomics NMR Fingerprinting for Discriminating Plant Metabolomes

4 Metabolomics NMR Profiling and Spectra Annotation

5 Application of 1H-NMR Profiling

6 New Challenges for 1H-NMR Metabolomics

7 Concluding Remarks

References

Chapter Two. Application of LC–MS and LC–NMR Techniques for Secondary Metabolite Identification

1 Introduction

2 Principal Secondary Metabolites in Plants

3 Hyphenated Chromatographic Techniques in Metabolite Identification

4 LC–NMR Techniques

5 Combination of LC–MS and LC–NMR in Metabolite Profiling

6 Conclusion

References

Chapter Three. Fast and Ultrafast Quantitative 2D NMR: Vital Tools for Efficient Metabolomic Approaches

1 Introduction

2 Why Quantitative 2D NMR?

3 Fast Conventional 2D NMR Approaches for Quantitative Analysis of Complex Mixtures

4 Going Faster

5 Conclusion

Acknowledgements

References

Chapter Four. Liquid Chromatography Coupled to Mass Spectrometry-Based Metabolomics and the Concept of Biomarker

1 Introduction

2 LC/MS-Based Metabolomics for Biomarker Discovery

3 Conclusion and Perspectives

Acknowledgements

References

Chapter Five. Potential of Fourier Transform Mass Spectrometry for High-Throughput Metabolomics Analysis

1 Introduction

2 Definition of Some Concepts Used in Mass Spectrometry

3 Principle of Fourier Transform Mass Spectrometers

4 Ion Production for Metabolite Analysis

5 FT-MS Applications for Complex Mixture Analysis

6 Metabolite Identification from HRMS Data

7 Conclusion

Acknowledgements

References

Chapter Six. Exploring Metabolome with GC/MS

1 Introduction

2 Sample Extraction for Metabolomic Analysis

3 GC/MS Profiling

4 Data Processing

5 Quantification

6 Applications

7 Conclusion

References

Chapter Seven. The Lipid World Concept of Plant Lipidomics

Abbreviations

1 Introduction

2 The Diversity of Plant Lipids

3 Lipid Extraction

4 Sample Storage

5 Principles of MS Lipid Analysis

6 Plant Lipid Analyses

7 Conclusion

Acknowledgements

References

Chapter Eight. Isotope Ratio Mass Spectrometry Technique to Follow Plant Metabolism: Principles and Applications of 12C/13C Isotopes

Abbreviations

1 Stable Isotopes at Natural Abundance

2 Definitions

3 Basics of Photosynthetic 12C/13C Discrimination

4 Practical Aspects of EA-IRMS Measurements

5 Compound-Specific IRMS Analyses

6 Major Advances in Metabolic Patterns Provided by IRMS Techniques

7 Conclusions and Perspectives

References

Chapter Nine. High-Throughput Biochemical Phenotyping for Plants

1 Introduction

2 Biochemical Phenotyping in Medicine, Agro Industry and Agriculture

3 Solutions for a Microplate Pipeline Dedicated to Plant Biochemical Phenotyping

4 Example of HT Biochemical Screen

5 Concluding Remarks

Acknowledgements

References

Chapter Ten. Two Computational Simplex Approaches to Graphical Highlighting Metabolic Phenotypes and Their Functional Origins: Correspondence Analysis and Weighted Metabolic Profiles Analysis

1 Introduction

2 Geometrical and Numerical Bases of Simplex Space

3 CA: Principle and Method

4 Weighted Metabolic Profiles Analysis

5 Application Example Combining CA and WMPA

References

Chapter Eleven. Strategies for Data Handling and Statistical Analysis in Metabolomics Studies

1 Introduction

2 General Characteristics of the Data

3 NMR Data Recording and Preprocessing: The Necessary Steps Prior to Statistical Analyses

4 LC/MS Data Recording and Preprocessing: The Necessary Steps Prior to Statistical Analyses

5 GC/MS Data Recording and Preprocessing: The Necessary Steps Prior to Statistical Analyses

6 Data Pretreatment

7 Basic Methods for Statistical Analysis: Possible Approaches

8 Reproducibility and Experimental Issues

9 Supervised Methods for Classification Problems: Potential for Statistical Bias

References

Chapter Twelve. Qualitative Modelling of Metabolic Networks

1 Introduction

2 Reconstruction, Storage and Sharing

3 Graph Formalism for Network Mining

4 Visualization of Metabolic Networks

5 Conclusion

References

Chapter Thirteen. Modelling Metabolic Networks—The Theories of Metabolism

1 Which Rate Equations for the Reactions of a Metabolic Network?

2 The Structure of Metabolic Network: The Stoichiometry Matrix

3 The Steady State

4 The Structure of Metabolic Network: The Elementary Flux Modes (efms)

5 Metabolic Control Analysis

6 Softwares and Tools for Kinetic Modelling

7 Conclusion: Protocol for the Study of Metabolic Networks at Steady State

References

Author Index

Subject Index

 
 
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