Molecular Microbiology Laboratory, 2nd Edition

Key Features

• Exposes students to the new molecular-based techniques
• Provides faculty with an authoritative, accessible resource for teaching protocols
• The only manual to incorporate writing exercises, presentation skills and tools for reading primary literature into the curriculum
• Based on a successful course for which the author won a teaching award

New to this Edition:
- Presents a real-world study of bacterial populations in the environment in the final experiment
- Provides an overview of molecular biology in a new review chapter
- Demonstrates how to design an experiment and how to interpret the results
- Covers grant proposal writing and how panels review proposals
- Presents guidance on public speaking and preparing PowerPoint presentations
- Includes tutorials on three widely used software packages

Description

Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles.

Readership

Appropriate for college sophomores through seniors in any of the life sciences, including microbiology, genetics, general biology, biochemistry, molecular biology, botany, zoology, etc. The experiments are also appropriate for properly equipped junior colleges and liberal arts institutions.

Molecular Microbiology Laboratory, 2nd Edition

Introduction
Experiment 1: Plasmid Purification and Restriction Mapping
Experiment 2: Affinity Purification of Histidine-Tagged FnbA Protein
Experiment 3: Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes
Experiment 4: Terminal Restriction Fragment Length Polymorphism Analysis of 16 S Ribosomal RNA Genes from Uncultured Bacterial Communities
Appendix A: Sample Laboratory Reports
Appendix B: Sample Proposals
Appendix C: Sample Tests
Appendix D: Guidance for Presentations
Appendix E: Revised Paragraph from J. Biol. Chem. 266: 3811-3814 (1991)
Appendix F: Abstract from Appl. Environ. Microbiol. 63: 2647-2653 (1997)
Appendix G: Sequence Editing Tutorial
Appendix H: Peak Scanner Tutorial
Appendix I: Adding Size Standards to Peak Scanner
Appendix J: PRIMER Tutorial
Appendix K: Checklist for Grading Laboratory Reports
Appendix L: Peer Review Checklist
Appendix M: Career Advice
Appendix N: Molecular Microbiology Laboratory Preparation Manual
Bibliography
Index