»
Protein Engineering for Therapeutics, Part B
 
 

Protein Engineering for Therapeutics, Part B, 1st Edition

 
Protein Engineering for Therapeutics, Part B, 1st Edition,K. Dane Wittrup,Gregory L. Verdine,ISBN9780123969620
 
 
Up to
25%
off
 

Methods in Enzymology

Wittrup   &   Verdine   

Academic Press

9780123969620

9780123972644

392

229 X 152

This volume of Methods in Enzymology is the companion to volume 502 and looks at Protein Engineering for Therapeutics

Print Book + eBook

USD 235.20
USD 392.00

Buy both together and save 40%

Print Book

Hardcover

In Stock

Estimated Delivery Time
USD 149.25
USD 199.00

eBook
eBook Overview

DRM-free included formats : EPUB, Mobi (for Kindle), PDF, EPUB, Mobi (for Kindle)

VST (VitalSource Bookshelf) format

USD 144.75
USD 193.00
Add to Cart
 
 

Key Features

  • Chapters provide an invaluable resource for academics, researchers and students alike
  • Iinternational board of authors
  • This volume is split into sections that cover subjects such as peptides, and scaffolds

Description

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Information about this author is currently not available.
Information about this author is currently not available.

Protein Engineering for Therapeutics, Part B, 1st Edition

Volume in series  

Preface

Stapled Peptides for Intracellular Drug Targets

1. Introduction

2. All-Hydrocarbon Stapled a-Helical Peptides

3. The Design of Stapled Peptides

4. Stapled Peptide Synthesis

5. Olefin Metathesis

6. N-terminal and Internal Modifications

7. Cleavage of Stapled Peptides from the Solid Support

8. Stapled Peptide Purification

9. Biophysical Characterization

10. Cell Permeability

11. In Vitro Target Interaction and Activity Assays

12. In Vivo Efficacy

13. Strategies for Stapled Peptide Optimization

14. Summary

Acknowledgments

Mapping of Vascular ZIP Codes by Phage Display

1. Introduction

2. Methods

3. Concluding Remarks and Perspectives

Acknowledgments

Engineering Cyclic Peptide Toxins

1. Introduction

2. Peptide Design

3. Peptide Synthesis

4. Structural Analysis

5. Stability Assays

6. Special Considerations for Cyclic Peptides

Acknowledgments

Peptide Discovery Using Bacterial Display and Flow Cytometry

1. Introduction

2. Protocols

3. Conclusions

Acknowledgments

Designed Ankyrin Repeat Proteins (DARPins)

1. Introduction

2. Applications of DARPins

3. Protocols for DARPins in Biomedical Applications

Acknowledgment

Target-Binding Proteins Based on the 10th Human Fibronectin Type III Domain (10Fn3)

1. Introduction

2. Library Design

3. Choice of Selection Platform

4. Phage Display, mRNA Display, and Yeast-Surface Display of 10Fn3-Based Libraries

5. Production

6. Conclusion

Acknowledgments

Anticalins

1. Introduction

2. Cloning and Expression of Lipocalins and Anticalins in E. coli

3. Construction of a Genetic Anticalin Library

4. Preparation and Selection of a Phage Display Library for Anticalins

5. Preparation and Selection of a Bacterial Surface Display Library for Anticalins

6. Colony Screening for Anticalins with Specific Target-Binding Activity

7. Screening for Anticalins with Specific Target-Binding Activity Using Microtiter Plate Expression in E. coli

8. Measuring Target Affinity of Anticalins in an ELISA

9. Measuring Target Affinity of Anticalins via Surface Plasmon Resonance

10. Application of Anticalins in Biochemical Research and Drug Development

T Cell Receptor Engineering

1. Introduction

2. Stability and Affinity Engineering of T Cell Receptors by Yeast Surface Display

3. Affinity Engineering and Selection of T Cell Receptors by T Cell Display

4. Expression, Purification, and Applications of Soluble scTv Proteins

5. Recipes for Media and Buffers

Acknowledgments

Engineering Knottins as Novel Binding Agents

1. Introduction

2. Knottins as Scaffolds for Engineering Molecular Recognition

3. Engineering Knottins by Yeast Surface Display

4. Knottin Library Construction

5. Screening Yeast-Displayed Knottin Libraries

6. Knottin production by chemical synthesis or recombinant expression

7. Cell binding assays

8. Summary

Acknowledgments

Practical Theoretic Guidance for the Design of Tumor-Targeting Agents

1. Introduction

2. What Molecular Size Is Best for Tumor Uptake?

3. Will Targeting Increase Nanoparticle Accumulation in a Tumor?

4. How Does Affinity Affect Biodistribution?

5. What Dose Is Necessary in Order to Overcome the “Binding Site Barrier”?

6. Conclusions

Reengineering Biopharmaceuticals for Targeted Delivery Across the Blood–Brain Barrier

1. Introduction

2. Blood–Brain Barrier Receptor-Mediated Transport and Molecular Trojan Horses

3. Reengineering Recombinant Proteins for Targeted Brain Delivery

4. Genetic Engineering of Expression Plasmid DNA Encoding IgG Fusion Proteins

5. Pharmacokinetics and Brain Uptake of IgG Fusion Proteins

6. CNS Pharmacological Effects of IgG Fusion Proteins

7. Immune Response Against IgG Fusion Proteins

8. Summary

Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells

1. Introduction

2. Methods

3. Conclusion

 
 
Free Shipping
Shop with Confidence

Free Shipping around the world
▪ Broad range of products
▪ 30 days return policy
FAQ

Contact Us