Volume 511: RNA helicases, 1st Edition

Key Features

• This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases
• The contributions in this volume cover the broad scope of methods in the research on these enzymes

Description

This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases.

RNA helicases, 1st Edition

1. Analysis of Duplex Unwinding by RNA Helicases Using Stopped Flow Fluorescence Spectroscopy

Andrea A. Putnam & Eckhard Jankowsky

2. Analyzing ATP Utilization by DEAD-box RNA Helicases Using Kinetic and Equilibrium Methods

Michael J. Bradley & Enrique M. De La Cruz

3. Oxygen Isotopic Exchange Probes of ATP Hydrolysis by RNA Helicases

David D. Hackney

4. Conformational Changes of DEAD-box Helicases Monitored by Single Molecule Fluorescence Resonance Energy Transfer

Alexandra Z. Andreou & Dagmar Klostermeier

5. RNA Catalysis as a Probe for Chaperone Activity of DEAD-box Helicases

Jeffrey P. Potratz & Rick Russell

6. Molecular Mechanics of RNA Translocases

Steve C. Ding & Anna Marie Pyle

7. Analysis of Helicase-RNA Interactions Using Nucleotide Analog Interference Mapping

Annie Schwartz, Makhlouf Rabhi, Emmanuel Margeat & Marc Boudvillain

8. Crystallization and X-ray Structure Determination of an RNA-dependent Hexameric Helicase

Nathan D. Thomsen & James M. Berger

9. Structural Analysis of RNA Helicases with Small-angle X-ray Scattering

Manja A. Behrens, Yangzi He, Cristiano L. P. Oliveira, Gregers R. Andersen, Jan Skov Pedersen & Klaus H. Nielsen

10. Analysis of Co-factor Effects on RNA Helicases

Crystal Young & Katrin Karbstein

11. Analysis of DEAD-box Proteins in mRNA Export

Ben Montpetit, Markus A. Seeliger & Karsten Weis

12. Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-mediated mRNA Decay

Francesca Fiorini, Fabien Bonneau & Hervé Le Hir

13. Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC)

Markus T. Bohnsack, David Tollervey & Sander Granneman

14. In Vivo Approaches to Dissecting the Function of RNA Helicases in Eukaryotic Ribosome Assembly

David C. Rawling & Susan J. Baserga

15. Analysis of RNA Helicases in P-bodies and Stress Granules

Angela Hilliker

16. DEAD box RNA Helicases as Transcription Co-factors

Frances V. Fuller-Pace & Samantha M. Nicol

17. DEAD-box RNA Helicases in Gram-positive RNA-decay

Peter Redder & Patrick Linder

18. RNA Helicases in Cyanobacteria: Biochemical and Molecular Approaches

George W. Owttrim

19. Determination of Host RNA Helicases Activity in Viral Replication

Amit Sharma & Kathleen Boris-Lawrie

20. Inhibitors of Translation Targeting Eukaryotic Translation Initiation Factor 4A

Regina Cencic, Gabriela Galicia-Vázquez & Jerry Pelletier

21. Identification and Analysis of Inhibitors Targeting the Hepatitis C Virus NS3 Helicase