»
»
Forensic DNA Biology
 
 

Forensic DNA Biology, 1st Edition

A Laboratory Manual

 
Forensic DNA Biology, 1st Edition,Kelly Elkins,ISBN9780123945853
 
 
 

  

Academic Press

9780123945853

9780123948335

224

235 X 191

A practical lab manual describing how to perform forensic DNA analysis

Print Book + eBook

USD 59.94
USD 99.90

Buy both together and save 40%

Print Book

Spiral bound

In Stock

Estimated Delivery Time
USD 49.95

eBook
eBook Overview

VST (VitalSource Bookshelf) format

DRM-free included formats : EPUB, Mobi (for Kindle), PDF

USD 49.95
Add to Cart
 
 

Key Features

* Provides practical step-by-step instruction on how to perform forensic DNA analysis
* Includes analysis of hair, presumptive testing of body fluids, human DNA typing and statistics
* Covers techniques such as pipetting, microscopy and DNA extraction
* Pre- and post-lab exercises and questions assist the reader in learning the material
* Report writing templates assure the reader learns real world crime lab procedure

Description

DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies will be used throughout and the author will provide an Instructor Manual and PowerPoint lecture slides.

Readership

Upper-level, undergraduate or graduate students majoring in forensic science, chemistry or biology, academia and newly practicing forensic scientists.

Kelly Elkins

Dr. Elkins is Assistant Professor of Chemistry at Towson University where she teaches in the Masters of Science in Forensic Chemistry Program. Previously, she was the Director of Forensic Science and Assistant Professor of Chemistry at Metropolitan State College of Denver. She has her Bachelor of Science in Biology, Bachelor of Arts in Chemistry and her PhD in Chemistry with an emphasis on Biochemistry. Focused on the Forensic DNA field, she has developed a network of contacts in crime laboratories throughout Colorado and the Four Corners region and looks forward to extending her network in the Baltimore/DC area.

Affiliations and Expertise

Assistant Professor of Chemistry, Chemistry Department and Forensic Chemistry Program, Towson University, Towson, Maryland, USA

Forensic DNA Biology, 1st Edition

Acknowledgements

About the Author

Welcome

Forensic DNA Biology: An Introduction

Biology Overview

Restriction Fragment Length Polymorphisms

Polymerase Chain Reaction

Short Tandem Repeats

Single Nucleotide Polymorphisms

Mitochondrial DNA

Known versus Questioned Samples

Why Study Forensic DNA Biology?

Laboratory Safety

Rules for a Safe Lab Environment

Avoiding Contamination Issues

Chapter 1. Pipetting

Objective

Safety

Materials

Background

Procedure

Questions

Graphing the Data Using Microsoft Excel (2003)

Equations

REFERENCES

Chapter 2. Serology

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 3. Sampling Biological Evidence for DNA Extraction

Objective

Safety

Materials

Background

Procedure: DNA Collection and Packaging

Questions

REFERENCES

Chapter 4. DNA Extraction

Objective

Safety

Materials

Recipes for Buffer and Solution Preparation

Background

Procedure

Question

REFERENCES

Chapter 5. Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCE

Chapter 6. Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 7. Determination of DNA Quantity by Fluorescence Spectroscopy

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 8. Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 9. Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico)

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 10. Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 11. Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 12. Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex Kit

Objective

Safety

Materials

Background

Procedure

Question

REFERENCES

Chapter 13. Computing Random Match Probability from DNA Profile Data Using Population Databases

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 14. Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 15. Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 16. Ribonucleic Acid (RNA) Extraction

Objective

Safety

Materials

Background

Procedure

Question

REFERENCES

Chapter 17. Y-STR Polymerase Chain Reaction (PCR) Deoxyribonucleic acid (DNA) Amplification and Typing

Objective

Safety

Materials

Background

Procedure

Question

REFERENCES

Chapter 18. Human Genetic Analysis: Paternity or Missing Persons Cases and Statistics

Objective

Safety

Materials

Background

Procedure

REFERENCES

Chapter 19. Low Copy Number Stochastic Results

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 20. Using in Silico Methods to Construct a Short-Tandem Repeat (STR) Deoxyribonucleic Acid (DNA) Sequence for Cloning

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCE

Chapter 21. Deoxyribonucleic Acid (DNA) Extraction from Botanical Material and Polymerase Chain Reaction (PCR) Amplification

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 22. Social, Ethical, and Regulatory Concerns

REFERENCES

Selected Forensic DNA Biology Case Studies

Index

Quotes and reviews

"This manual presents a collection of novel laboratory experiments intended for advanced undergraduate students and those new to the field of forensic DNA biology and DNA typing."--Evidence Technology Magazine, July-August 2013

 
 
Free Shipping
Shop with Confidence

Free Shipping around the world
▪ Broad range of products
▪ 30 days return policy
FAQ

Contact Us