Volume 504: IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS, 1st Edition

Key Features

Description

This volume of Methods in Enzymology is the first of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS, 1st Edition

Part A: Techniques

1. Laser Induced Radiation Microbeam Technology and Simultaneous Real-time Fluorescence Imaging in Live Cells

Stanley W. Botchway, Pamela Reynolds, Anthony W. Parker, Peter O’Neill

2. A cell biologist’s guide to high resolution imaging

Graeme Ball, Richard M Parton, Russell S Hamilton and Ilan Davis

3. Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo

André E.P. Bastos, Silvia Scolari, Martin Stöckl, Rodrigo F.M. de Almeida

4. Detecting and Tracking Non-Fluorescent Nanoparticle Probes in Live Cells

Gufeng Wang and Ning Fang

5. Fluorescence lifetime microscopy of tumor cell invasion, drug delivery and cytotoxicity

Gert-Jan Bakker, Volker Andresen, Robert M. Hoffman, Peter Friedl

6. Measuring membrane protein dynamics in neurons using Fluorescence recovery after photobleach (FRAP)

Inmaculada M González-González, Frédéric Jaskolski, Yves Goldberg, Michael C. Ashby and Jeremy M. Henley

7. Distinctive characteristics of Madeira wine regarding its traditional winemaking and modern analytical methodologies

Rosa Perestrelo, Francisco Albuquerque, Sílvia M. Rocha, José S. Câmara

8. Green to red photo-convertible mEosFP aided live imaging in plants

Jaideep Mathur, Sarah Griffiths, Kiah Barton and Martin H. Schattat

9. Methods for Cell and Particle Tracking

Erik Meijering, Oleh Dzyubachyk, Ihor Smal

10. Correlative light-electron microscopy: a potent tool for the imaging of rare or unique cellular and tissue events and structures

Alexander A. Mironov and Galina V. Beznoussenko

11. Optical Techniques for Imaging Membrane Domains in Live Cells (Live Cell Palm of Protein Clustering)

Dylan M. Owen, David Williamson, Astrid Magenau, and Katharina Gaus

12. Single live cell topography and activity imaging with the Shear-Force-Based constant-distance scanning electrochemical microscope

Albert Schulte, Michaela Nebel and Wolfgang Schuhmann

13. Visualization of TGN-endosome trafficking in mammalian and drosophila cells

Satoshi Kametaka and Satoshi Waguri

14. Live cell imaging with chemical specificity using dual frequency CARS microscopy.

Iestyn Pope, Wolfgang Langbein, Paola Borri and Peter Watson

15. Imaging intracellular protein dynamics by spinning disk confocal microscopy

Samantha Stehbens, Hayley Pemble, Lindsay Murrow and Torsten Wittmann

Part B: Tools

16. Visualizing Dynamic Activities of Signaling Enzymes using genetically encodable FRET-based biosensors: from designs to applications

Xin Zhou, Katie J. Herbst, Jin Zhang

17. Live-cell Imaging of Aquaporin-4 Supramolecular Assembly and Diffusion

A.S. Verkman, Andrea Rossi and Jonathan M. Crane

18. Coiled-coil tag-probe labeling methods for live-cell imaging of membrane receptors

Yoshiaki Yano, Kenichi Kawano, Kaoru Omae, and Katsumi Matsuzaki

19. Monitoring Protein Interactions in Living Cells with Fluorescence Lifetime Imaging Microscopy

Yuansheng Sun, Nicole M. Hays, Ammasi Periasamy, Michael W. Davidson, and Richard N. Day

20. Open Source Tools for Fluorescent Imaging

Nicholas A. Hamilton

21. Nanoparticle PEBBLE sensors in live cells
    
    Yong-Eun Koo Lee and Raoul Kopelman