Volume 505: IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS, 1st Edition

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Description

This volume of Methods in Enzymology is the second of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS, 1st Edition

Part A: Organelles and Cellular Structure

1. Monitoring Cytoskeletal Dynamics in Living Neurons using Fluorescence Photoactivation

Anne Gauthier-Kemper, Carina Weissmann, Hans-Jürgen Reyher, and Roland Brandt

2. Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays

Roy Edward

3. Methods to measure actin treadmilling rate in dendritic spines

Mikko Koskinen, Enni Bertling, and Pirta Hotulainen

4. Live Cell Imaging of Clathrin Coats

Comert Kural and Tom Kirchhausen

5. Imaging the Mitotic Spindle

Paul S. Maddox, Anne-Marie Ladouceur, Rajesh Ranjan, Jonas Dorn, Hery Ratsima, Damien D’Amours, and Amy S. Maddox

6. Modeling of spatial intracellular signaling events in neurons

Wendy C. Wenderski and Susana R. Neves

7. Imaging chromosome dynamics in meiosis in plants

Moira J. Sheehan, and Wojciech P. Pawlowski

8. Analysing lysosomes in live cells

Paul R. Pryor

9. Detection and Quantification of Biomolecular Association in Living Cells using Single Molecule Microscopy

Mario Brameshuber,and Gerhard J. Schütz

10. Visualizing cardiac ion channel trafficking pathways

James W. Smyth and Robin M. Shaw

11. Live Cell Imaging of the Cytoskeleton

Eve G. Stringham, Nancy Marcus-Gueret, Laura Ramsay and Kristopher L. Schmidt

12. Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms

Naoki Watanabe

13. Analysis of cell dispersion and migration by video-microscopy

Bonnomet Arnaud, Terryn Christine, Cutrona Jérôme, Jonquet Antoine, Birembaut Philippe, and Zahm Jean-Marie

Part B: Molecules and Ions

14. Real Time Qualitative and Quantitative Glut4 Translocation Assay

Maleppillil Vavachan Vijayakumar and Manoj Kumar Bhat

15. Advanced imaging of cellular signalling events

JMarek Cebecauer, Jana Humpolícková and Jerémie Rossy

16. Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy

Subhasri Ghosh, Suvrajit Saha, Debanjan Goswami, Sameera Bilgrami and Satyajit Mayor

17. Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells

Harsha E. Rajapakse and Lawrence W. Miller

18. Imaging of corticosteroid receptors in live cells

Mayumi Nishi

19. Investigating second messenger signaling in vivo

Rüdiger Rudolf, Mathias Hafner, and Marco Mongillo

20. Probes for Intracellular RNA Imaging In Live Cells

Philip J. Santangelo, Eric Alonas, Jeenah Jung, Aaron W. Lifland, and Chiara Zurla

21. Imaging the Glycome in Living Systems

Boyangzi Li, Feiyan Mock and Peng Wu

22. Intracellular Magnesium Detection by Fluorescent Indicators

Valentina Trapani, Monika Schweigel-Röntgen, Achille Cittadini and Federica I. Wolf

23. Illuminating mobile zinc with fluorescence: From cuvettes to live cells and tissues

Zhen Huang and Stephen J. Lippard

24. Quantitative fluorescent live cell imaging of intracellular Ca2+ and H+ ions in malaria parasites

          Petra Rohrbach