Lipids, 1st Edition

Lipids, 1st Edition,Gilbert Di Paolo,Markus R. Wenk,ISBN9780123864871

Methods in Cell Biology

Di Paolo   &   Wenk   

Academic Press




235 X 191

Lipids are a broad group of naturally occurring molecules, and this volume of Methods in Cell Biology covers such areas as membrane structure and dynamics, imaging, and lipid protein interactions.

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Key Features

  • Covers such areas as membrane structure and dynamics, imaging, and lipid protein interactions
  • An essential tool for researchers and students alike
  • International authors
  • Renowned editors


Lipids are a broad group of naturally occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The main biological functions of lipids include energy storage, as structural components of cell membranes, and as important signaling molecules.
This volume of Methods in Cell Biology covers such areas as Membrane structure and dynamics, Imaging, and Lipid Protein Interactions. It will be an essential tool for researchers and students alike.


Researchers and students in cell, molecular and developmental biology

Information about this author is currently not available.
Information about this author is currently not available.

Lipids, 1st Edition

Membrane Dynamics and Reconstitution Assays:

  1. Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding
  2. Min Wu and Pietro De Camilli

  3. Studying lipids involved in the endosomal pathway
  4. Christin Bissig, Shem Johnson, and Jean Gruenberg

  5. Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking
  6. Jean-Baptise Manneville, Cécile Leduc, Benoit Sorre, and Guillaume Drin

  7. Reconstituting Multivesicular Body Biogenesis with purified components
  8. Thomas Wollert

  9. Approaches to the study of Atg8-mediated membrane dynamics in vitro
  10. Anjali Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, and Thomas J. Melia

  11. Reconstitution assay system for ceramide transport with semi-intact cells
  12. Keigo Kumagai, Masahiro Nishijim, Kentaro Hanada

  13. Visualizing mitochondrial lipids and fusion events in mammalian cells
  14. Huiyan Huang and Michael A. Frohman

    Lipid Metabolism and Signaling:

  15. Targeted and non-targeted analysis of membrane lipids using mass spectrometry
  16. Xue Li Guan and Markus R. Wenk

  17. Modulation of host phosphoinositide metabolism during Salmonella invasion by the type III secreted effector SopB
  18. Bernhard Roppenser, Sergio Grinstein and John H. Brumell

  19. Acute Manipulation of Phosphoinositide Levels in Cells
  20. Belle Chang-Ileto, Samuel G. Frere, and Gilbert Di Paolo

  21. Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins
  22. Marnix Wieffer, Volker Haucke and Michael Krauß

  23. Phosphoinositides at the neuromuscular junction of Drosophila melanogaster: a genetic approach.
  24. Jan R. Slabbaert Thang Manh Khuong and Patrik Verstreken

  25. Devising powerful Genetics, Biochemical and Structural tools in the Functional Analysis of Phosphatidylinositol Transfer Proteins (PITPs) across diverse species
  26. Jim Davison, Vytas A. Bankaitis and Ratna Ghosh

  27. Genome-wide screens for gene products regulating lipid droplet dynamics.
  28. Weihua Fei and Hongyuan Yang


  29. The Three Dimensionality of Cell Membranes: Lamellar to Cubic Membrane Transition as Investigated by Electron Microscopy
  30. Ketpin Chong and Yuru Deng

  31. Quantitative imaging of lipid metabolism in yeast: from 4D analysis to high content screens of mutant libraries
  32. Heimo Wolinski, Kristian Bredies and Sepp D. Kohlwein

  33. Analysis of cholesterol trafficking with fluorescent probes
  34. Frederick R. Maxfield and Daniel Wüstner

  35. Fluorescence Correlation Methods for Imaging Cellular Behavior of Sphingolipid-Interacting Probes
  36. Rachel Kraut, Nirmalya Bag, and Thorsten Wohland

  37. Monitoring Phospholipid Dynamics during Phagocytosis: Application of Genetically-Encoded Fluorescent Probes
  38. Helen Sarantis and Sergio Grinstein

  39. Genetically encoded probes for phosphatidic acid

          Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J.Grant and Nicolas Vitale

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