Essential Zebrafish Methods: Genetics and Genomics, 1st Edition

Key Features

* Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors
* Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error

Essential Zebrafish Methods: Genetics and Genomics, 1st Edition

1. Haploid Screens and Gamma-Ray Mutagenesis (Charline Walker)
2. Positional Cloning of Mutated Zebrafish Genes (Will Talbot)
3. The Zebrafish Genome (John Postlethwait)
4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish (Adam Amsterdam)
5. Genetic Screens for Maternal-Effect Mutations (Francisco Pelegri)
6. Behavior Screening Assays in Zebrafish (Herwig Baier)
7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish (Cecelia B. Moens)
8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element (Koichi Kawakami)9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish (Charles Lee)
10. The Zon Laboratory Guide to Positional Cloning in Zebrafish (Leonard I. Zon)
11. Sleeping Beauty Transposon for Efficient Gene Delivery (Stephen C. Ecker)
12. Transgene Manipulation in Zebrafish by Using Recombinases (Gary W. Stuart)
13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI (Joachim Wittbrodt)
14. Cloning Zebrafish by Nuclear Transfer (Shuo Lin)
15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening (Christine Thisse)
16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish (Bill Trevarrow)
17. Common Diseases of Laboratory Zebrafish (Jennifer L. Matthews)
18. Zebrafish Sperm Cryopreservation (Thomas Look)