Guide to Protein Purification

Guide to Protein Purification, 2nd Edition

Guide to Protein Purification, 2nd Edition,Richard Burgess,Murray Deutscher,ISBN9780123749789

Methods in Enzymology

Burgess   &   Deutscher   

Academic Press



229 X 152

An essential reference for any researcher involved in protein purification, this volume is a comprehensive collection of methods necessary for purifying, characterizing, and handling proteins and enzymes.

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Key Features

  • Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology
  • Assembles chapters on both common and less-common relevant techniques
  • Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process


The 2eof this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today.


Researchers, professors, and students of biochemistry, pharmacology, genetics, molecular biology and cell biology

Information about this author is currently not available.

Murray Deutscher

Affiliations and Expertise

University of Connecticut Health Center, Farmington, Connecticut, U.S.A.

Guide to Protein Purification, 2nd Edition

  1. Why Purify Enzymes?
  2. Strategies and Considerations for Protein Purifications (Linn)
  3. Bioinformatics in Planning a Protein Purification (Burgess)
  4. Preparing a Protein Purification Summary Table (Burgess)
  5. Setting up a Laboratory (Deutscher)
  6. Buffers: Principle and Practice (Blanchard)
  7. Measurement of Enzyme Activity (Harris)
  8. Quantitation of Protein (Bailey and Noble)
  9. Maintaining Protein Stability (Deutscher)
  10. Strategy for Choosing and Expression System (Brondyk)
  11. Bacterial Expression Systems (Collart)
  12. Yeast Expression Systems (Cregg)
  13. Baculovirus-Insect Cell Expression Systems (Jarvis)
  14. Mammalian Expression Systems (Geisse and Knopf)
  15. Expression of Tagged Proteins (Melhorta)
  16. Re-folding of Solubilized Inclusion Body Proetin (Burgess)
  17. Preparation of Biological Extract (Grabski)
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