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Single Molecule Tools, Part A: Fluorescence Based Approaches
 
 

Single Molecule Tools, Part A: Fluorescence Based Approaches, 1st Edition

 
Single Molecule Tools, Part A: Fluorescence Based Approaches, 1st Edition,Nils Walter,ISBN9780123749543
 
 
 

Methods in Enzymology

N Walter   

Academic Press

9780123749543

9780080959276

528

229 X 152

New volume featuring tried-and-true protocols meant to guide researchers through single-molecule methods

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Key Features

    * Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools
    * Methods included are useful to both established researchers and newcomers to the field
    * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

    Description

    Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.

    Readership

    Biochemists, geneticists, molecular biologists, cell biologists, and biophysicists.

    Nils Walter

    Single Molecule Tools, Part A: Fluorescence Based Approaches, 1st Edition

    1. Watching single DNA replication loops under flow extension
    2. Star polymer surface passivation for single molecule detection
    3. Ultrahigh resolution detection of single active motor proteins in live cells
    4. Molecules and Methods for Superresolution Imaging
    5. Aqueous nanodroplets for studying single molecules
    6. High-speed atomic force microscopy techniques for visualizing dynamic behavior of biological macromolecules
    7. Single-Biomolecule Spectroscopy Using Microfluidic Platforms
    8. DNA Looping Kinetics Analyzed by Tethered Particle Microscopy
    9. Single molecule observation of proteins in vivo
    10. DNA curtains as a high-throughput approach to single molecule imaging
    11. Single-molecule enzymology of protein synthesis
    12. Single molecule fluorescence studies of intrinsically disordered proteins
    13. Nanovesicle trapping for studying transient protein-protein interactions by single molecule FRET
    13. Tracking single motor proteins in the cytoplasm of mammalian cells
    14. Conformational States of F1-ATPase by Single-Molecule Rotation
    15. Single Molecule Sequencing by Fluorescence Imaging
    16. Real-Time DNA Sequencing from Single Polymerase Molecules
    17. Micropatterning and single molecule imaging for quantitative analysis of protein-protein interactions in living cells
    18. Probing virus-receptor interactions by atomic force spectroscopy
    19. Single-Molecule Fluorescence Spectroscopy of Cytochrome P450 in Nanodiscs
    20. Analysis of complex single molecule FRET time traces
    21. Application of super-resolution imaging to single particle tracking in nanotechnology
    22. Scanning FCS for the characterization of protein dynamics in live cells
    23. Single mRNA molecule tracking in live cells
    24. Single-molecule high-resolution colocalization (SHREC) or Single-molecule optical-trap analyses of protein structure
    25. Nanopore force Spectroscopy tools for analyzing single bio-complexes
    26. Use of plasmon coupling to reveal DNA dynamics at the single molecule level
    27. Fluorescence-force spectroscopy
    28. Multiplexed single mRNA imaging in fixed cells
    29. Size-Minimized Quantum Dots for Single-Molecule and Intracellular Imaging
    30. The ABEL trap
    31. An optical torque wrench
    32. Determining the Stoichiometry of Protein Hetero-complexes in Living Cells with Fluorescence Fluctuation Spectroscopy
    33. Fluorescent Visualization of Single Protein-DNA Complexes
    34. Direct Measurement of Tertiary Contact Cooperativity in RNA Folding by single molecule FRET
    35. Nanometer-localized multiple single-molecule (NALMS) 36. Multiparameter single molecule fluorescence detection with applications to FRET
    37. Single-particle tracking-photoactivated localization microscopy (sptPALM) within live cells
    38. Site-specific incoporation of fluorescent probes into RNA polymerase
    39.Quantitative single-molecule imaging by confocal laser scanning microscopy
    40. Studies of DNA-replication at the single molecule level using magnetic tweezers
    41. RNA labeled for single molecule FRET analysis from ligation with T4 RNA ligases
    42. Combining optical tweezers, single-molecule fluorescence microscopy and microfluidics for studying reversible protein-DNA interactions
    43. How dwell time distributions and other such observables in single molecule analysis can be used to extract information from molecular systems

     
     
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