Chemokines, Part B

Chemokines, Part B, 1st Edition

Chemokines, Part B, 1st Edition,Tracy Handel,Damon Hamel,ISBN9780123749079

Methods in Enzymology

Handel   &   Hamel   

Academic Press




229 X 152

The second installment of Tracy Handel and Damon Hamel's new, two-volume treatment on chemokines and chemokine receptors.

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Key Features

Along with its companion volume, provides a comprehensive overview of chemokine methods, specifically as related to potential disease therapy

Gathers tried, tested, and trusted methods and techniques from top players in chemokine research

Provides an essential reference for researchers in the field


The understanding of chemokines, the proteins that control the migration of cells, and their receptors, is critical to the study of causes and therapies for a wide range of human diseases and infections, including certain types of cancer, inflammatory diseases, HIV, and malaria. This volume, focusing on chemokine structure and function, as well as signaling, and its companion volume (Methods in Enzymology volume 461, focusing on chemokines as potential targets for disease intervention) provide a comprehensive overview and time-tested protocols in this field, making it an essential reference for researchers in the area.


Researchers in cell, molecular and developmental biology; biochemists pharmacologists, and geneticists

Tracy Handel

Professor, Skaggs School of Pharmacy and Pharmaceutical Sciences and Department of Pharmacology, School of Medicine, University of California, USA

Affiliations and Expertise

Skaggs School of Pharmacy and Pharmaceutical Sciences and Department of Pharmacology School of Medicine, University of California, USA

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Chemokines, Part B, 1st Edition

1. A Novel Chemokine-mediated In Vivo T Cell Recruitment Assay for Structure Function Studies
(Andrew D. Luster and Gabriele Campanella)
2. Tyrosine sulfation of HIV-1 coreceptors and other chemokine-binding receptors (Micheal R. Farzan)
3. Lymphotactin Structural Dynamics (Brian F. Volkman)
4. Regulation of chemokines by posttranslational modifications (Paul Proost )
5. Proteolytic Processing of Chemokines: Mass Spectrometry, Biochemistry, Neoepitope Antibody and Functional Characterization (Christopher Overall and Amanda Starr)
6. Multiple approaches to study Chemokine Receptor Homo and Heterodimerization (Mario Mellado)
7. Heterodimerization of Chemokine Receptors with Other GPCRs (Graeme Milligan and Shirley Appelbe)
8. Homo and Hetero-Oligomerization of Chemokines (Tracy Handel, Damon Hamel, Ariane Jansma)
9. Interactions of Chemokine with Glycosaminoglycans (Amanda Proudfoot)
10. Interactions of Chemokine with Glycosaminoglycans (Andreas J. Kungl)
11. Structures of Chemokine:Chemokine Binding Protein Complexes (Daved Fremont)
12. Activation Mechanisms of Chemokine Receptors (Mette Rosenkilde)
13. Modeling Small Molecule Compound Binding to Receptors (Richard Horuk, James Pease, N. Vaidehi)
14. Spectroscopic Approaches to Study Conformational Changes in GPCRs (David Farrens)
15. Plasmon Resonance Methods in GPCR Signaling (Victor Hruby, Isabel Alves, Gordon Tollin)
16. Flow Cytometric Analyses of GPCR Interactions or Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics (Larry Sklar)
17. Expression and Functional Reconstitution of CCR1 (Tracy Handel and Samantha Allen, Damon Hamel)
18. Characterization of the Interactions of Chemokines with Glycosaminoglycans by Fourier Transform Ion Cyclotron Resonance (Julie A. Leary)
19. In vitro assays for studying chemokine-triggered lymphocyte transendothelial migration under physiological flow conditions (Ronen Alon and Francis Lin)
20. Microfluidic device (Eugene Butcher)
21. Two-photon imaging and quantitative analysis of leukocyte migration in vivo (Mark Miller and Jenna Cash)
22. Zymosan induced peritonitis as a model for studying chemokines and inflammatory mediators in vivo (David Greaves)
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