Volume 115: Microtubules, in vitro, 1st Edition

Microtubules, in vitro, 1st Edition

Section1: Isolation, Biochemistry and Characterization of Antibodies and Isotypes.

1.  Preparation of Microtubule Protein and Purified Tubulin from Bovine Brain by Cycles of Assembly and Disassembly and Phosphocellulose Chromatography

Herbert P. Miller and Leslie Wilson

2. Measurement Of Ligand Binding To Tubulin By Sulfhydryl Reactivity

Adrian Begaye and Dan L Sackett

3. Isolating Tubulin From Nonneural Sources

Dan L. Sackett, Karl A. Werbovetz and Naomi S. Morrissette

4. Characterization of Anti-b -Tubulin Antibodies

Anthony J. Spano* and Anthony Frankfurter

5. Expression profiling of tubulin isotypes and microtubule interacting proteins using real-time polymerase chain reaction

Sharon Lobert, Laree Hiser and John J. Correia

6. Nondenaturing Electrophoresis As A Tool To Investigate Tubulin Complexes

Mónica López-Fanarraga, Gerardo Carranza, Raquel Castaño, Sofia Nolasco, J. Avila and J.C. Zabala

7. Mass spectrometry analysis of c-terminal posttranslational Modifications of tubulins

Redeker, Virginie

8. Methods in Tubulin Proteomics

Leah M. Miller, Hui Xiao, Berta Burd, Susan Band Horwitz, Ruth Hogue Angeletti, Pascal Verdier-Pinard

Section 2: Microtubule Structure and Dynamics

9.  Cryo-EM Studies of Microtubule Structural Intermediates and Kinetochore-Microtubule Interactions

Eva Nogales, Vincent H. Ramey and Hong-Wei Wang

10. High resolution imaging of microtubules and cytoskeleton structures by Atomic Force Microscopy

Loic Hamon, Patrick A. Curmi, David Pastré

11. Holly V. Goodson and Ivan V. Gregoretti

Using computational modeling to understand microtubule dynamics: a primer for cell biologists

12. Analysis of Dynamic Instability of Steady State Microtubules In Vitro by Video-Enhanced Differential Interference Contrast Microscopy with an Appendix by Emin Oroudjev

Mythili Yenjerla, Manu Lopus and Leslie Wilson

13. Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers

Blake D. Charlebois, Henry T. Schek III, Alan J. Hunt

14. Microtubule Dynamics Reconstituted in-vitro and Imaged y Single-Molecule Fluorescence Microscopy

Christopher Gell, Volker Bormuth, Gary J. Brouhard, Daniel N. Cohen, Stefan Diez, Claire T. Friel, Jonne Helenius, Bert Nitzsche, Heike Petzold, Jan Ribbe, Erik Schäffer, Jeffrey H. Stear, Anastasiya Trushko, Vladimir Varga, Per O. Widlund, Marija Zanic, and Jonathon Howard

15. Studying kinesin motors by optical 3D-nanometry in gliding motility assays

Bert Nitzsche, Volker Bormuth, Corina Bräuer, Jonathon Howard, Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow, Stefan Diez

Section 3: Drugs

16. Analysis of tubulin oligomers by Analytical Ultracentrifugation.

J.J. Correia

17.  Determination of Drug Binding to Microtubules in vitro

Jennifer A. Smith and Mary Ann Jordan

18. Fluorescence Spectroscopic Methods to Analyze Drug-Tubulin Interactions

Bhabatarak Bhattacharyya, Sonia Kapoor and Dulal Panda

19. A Tubulin polymerization micro-assay used to compare ligand efficacy

Ashley Davis, Sarah Martinez, Daniel Nelson and Kim Middleton.

20. Fluorescent Taxoid Probes for Microtubule Research

Isabel Barasoain, J. Fernando Díaz, and José M. Andreu

21.  The binding of vinca domain agents to tubulin: structural and biochemical studies

Anthony Cormie, Marcel Knossow, Chunguang Wang and Benoît Gigant

Section 4: Interactions with Motors and MAPs

22. Probing Interactions of Tubulin with Small Molecules, Peptides and Protein Fragments by Solution Nuclear Magnetic Resonance

Marie-Jeanne Clément, Philippe Savarin, Elisabeth Adjadj, André Sobel, Flavio Toma, Patrick A. Curmi

23. Microtubule and MAPs: thermodynamics of complex formation by AUC, ITC, fluorescence and NMR

François Devred, Pascale Barbier, Daniel Lafitte, Isabelle Landrieu, Guy Lippens and Vincent Peyrot

24. Quantitative Analysis of MAP Mediated Regulation of Microtubule Dynamic Instability In Vitro - Focus on Tau

Erkan Kiris, Donovan Ventimiglia and Stuart C. Feinstein

25. Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy (FPM)

Hernando Sosa, Ana B. Asenjo and Erwin J.G. Peterman

26. Multiple color single molecule TIRF imaging and tracking of MAPs and motors

Jennifer L. Ross and Ram Dixit

27. Dixit and Ross - final submitted 11/4 & lost - resubmitted 1/8/10
28. Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor and +TIP dynamics

Peter Bieling, Ivo A. Telley, Christian Hentrich, Jacob Piehler and Thomas Surrey

Section 5: Functional Extracts and Force Measurements

29. Quantitative Characterization of filament dynamics by single-molecule lifetime measurements.

Leonid A. Mirny and Daniel J. Needleman

30. Extracting the mechanical properties of microtubules from thermal fluctuation measurements on an attached tracer particle.

Katja M. Taute, Francesco Pampaloni, Ernst-Ludwig Florin

31. In vitro assays to study force generation at dynamic microtubule ends

Liedewij Laan and Marileen Dogterom

32. Reconstitution and functional analysis of kinetochore subcomplexes

Daniel R. Gestaut, Jeremy Cooper, Charles L. Asbury, Trisha N. Davis and Linda Wordeman

33. In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces

Ekaterina L. Grishchuk and Fazly I. Ataullakhanov