Microtubules, in vitro, 2nd Edition

Microtubules, in vitro, 2nd Edition,John Correia,Leslie Wilson,ISBN9780124077577

Methods in Cell Biology

Correia   &   Wilson   

Academic Press




235 X 191

2e of the Methods in Cell Biology volume on microtubules

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Key Features

  • Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research
  • Combines classical approaches and modern technologies
  • Presents easy-to-use protocols and thorough background information, compiled by leaders in the field


There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.


Researchers and students in cell, molecular and developmental biology

John Correia

Affiliations and Expertise

University of Mississippi Medical Center, Jackson, USA

View additional works by John J. Correia

Leslie Wilson

Affiliations and Expertise

University of California, Santa Barbara, U.S.A.

View additional works by Leslie Wilson

Microtubules, in vitro, 2nd Edition

  1. Isolation of Antarctic fish tubulin and MAPs
    H.William Detrich
  2. Making, isolating and characterizing tubulin alpha antibodies
    Anthony Spano and Tony Frankfurter
  3. Techniques for labeling tubulin and drugs
    Susan Bane
  4. Single-particle 3D reconstruction methods for decorated and undecorated microtubules
    Charles Sindelar
  5. A new microtubule gliding assay and analysis of microtubule persistence length
    Douglas S. Martin
  6. Characterization of gamma tubulin
    Berl R. Oakley
  7. Biochemical and Structural Studies of the doublecortin family of MAPs
    Carolyn A. Moores, Franck Fourniol and Anne Houdusse
  8. Detection and quantification of microtubule detachment from centrosomes and spindle poles
    Fernando R.Cabral
  9. Regulation of tubulin expression by micro RNAs: Implications for Drug Resistance
    Sharon Lobert Mary E. Graichen
  10. A portable magnetic tweezers device to visualize three-dimensional deformation of reconstituted microtubule networks
    Megan Valentine
  11. Mitochondrial/microtubule interactions - mmbp1
    Phong Tran
  12. Going Solo: Measuring the motions of microtubules with an in vitro assay for TIRF microscopy
    Kris D. Leslie and Neils Galjart
  13. Analysis of Microtubules and Motors in Isolated Axoplasm from the Squid Giant Axon
    Scott T. Brady
  14. Analysis of drug binding to microtubules and steady state microtubule growth/treadmilling by use of a sedimentation assay in vitro
    Leslie Wilson, Herbert P. Miller and Manu Lopus
  15. Specific recombinant antibody technology for the study of microtubule dynamics and regulatory proteins
    Franck Perez
  16. Tubulin-specific Chaperones: Components of a Molecular Machine that Assembles the aB ¿Heterodimer
    Nicholas J. Cowan and Guoling Tian
  17. G-protein tubulin interactions
    Mark M. Rasenick
  18. Katanin, a microtubule severing enzyme: expression, purification, and biophysical characterization
    Jennifer L. Ross, Juan Daniel Diaz-Valenica and Megan Bailey
  19. Measuring tubulin polymerization in vitro - comparison of methods and possible artifacts
    Dan Sackett
  20. Imaging microtubules in living plant cells
    Jessica Regan Lucas
  21. How to use which antibody known in the field, what to expect from this antibody and how semi-quantitative data can be reliably obtained with antibody staining
    Cartsen Janke and Maria Magdalena Magiera
  22. Preparation of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences
    Jose M. Andreu
  23. Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry
    François Devred, P.O. Tsvetkov, P. Barbier, G. Breuzard and V. Peyrot
  24. TBC
    Gaudenz Danuser
  25. Characterization of the mechanism of action of the covalent-binding microtubule-stabilizing agent zampanolide
    Jessica Field, Peter Northcote, John Miller, Karl-Heinz Altmann and José Fernando Díaz
  26. Studying kinetochore-fibre ultrastructure using correlative light-electron microscopy
    Liam P. Cheeseman
  27. Fluorescence-based Assays for Microtubule Structure
    Gary J. Brouhard and Susan Bechstedt
  28. Yeast Tubulin-structure-function analysis
    Mohan L. Gupta
  29. Microtubule Binding SIm to simulate binding of proteins to polymers under different binding models
    Holly Goodson

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