Introduction to Electron Microscopy for Biologists

Introduction to Electron Microscopy for Biologists, 1st Edition

Introduction to Electron Microscopy for Biologists, 1st Edition,Terry Allen,ISBN9780123743206

Methods in Cell Biology

T Allen   

Academic Press




235 X 191

This volume demonstrates how electron microscopy contributes to knowledge about biological structural information.

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Key Features

*Basic techniques in transmission and scanning electron microscopy
*Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton
*Discussion on electron microscopy of viruses and virus-cell interactions


This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).


Researchers in all fields of biology who use electron microscopy in their lab and studies.

Terry Allen


Affiliations and Expertise

Paterson Institute, University of Manchester, UK

Introduction to Electron Microscopy for Biologists, 1st Edition

PART ONE. Exploring the Organisation of the Cell by Electron Microscopy

Basic Transmission and Scanning Electron Microscopy

1. Scanning Electron Microscopy at the sub-cellular level-Terry Allen

2 Electron Probe X-ray microanalysis for the study of cell physiology.
E. Fernandez-Segura and A.Warley

3 Preparation of cells and tissues for Immuno EM
Paul Webster, Heinz Schwarz and Gareth Griffiths

4 Fixation, Freezing and Embedding for Genetically Altered Tissue
Heinz Schwarz

5 Combined video fluorescence and 3-D electron microscopy
Alexander A. Mironov, Roman S. Polishchuk and Galina V. Beznoussenko

6. From Live-cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common Label
Sheona P Drummond and Terry D Allen

7 Immunolabelling for High Resolution Scanning Electron Microscopy .- Martin Goldberg

8 Immunogoldlabeling of Thawed Cryosections
Peter J. Peters and Jason Pierson

9. Close-to-Native Ultrastructural Preservation by High Pressure Freezing
Dimitri Vanhecke, Werner Graber, and Daniel Studer

10. High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips
Siegfried Reipert and Gerhard Wiche

11 Freeze-fracture Cytochemistry in Cell Biology
Nicholas J. Severs and Horst Robenek

PART TWO. Electron Microscopy of Specific Cellular Structure
The Cell Membrane

12 The Plasma membrane cytoskeleton Akihiro Kusumi

The Cytoskeleton

13. Correlated Light and Electron Microscopy of the Cytoskeleton
Sonja Auinger and J.Victor Small

14 Electron microscopy of intermediate filaments: Teaming up with atomic force and confocal laser scanning microscopy
Laurent Kreplak, Karsten Richter, Ueli Aebi and Harald Herrmann

15. Studying Microtubules by Electron Microscopy
Carolyn Moores

Cytoplasm, Extracellular Matrix and Cell Junctions

16. Endoplasmic reticulum and Golgi Complex.
John Lucoqc

17. Visualization of Dynamins
Jason A. Mears and Jenny E. Hinshaw

18. Electron Microscopy of Collagen Fibril Structure In Vitro And In Vivo including Three-Dimensional Reconstruction
Tobias Starborg, Yinhui Lu, Karl E. Kadler and David F. Holmes

19 Visualisation of Desmosomes in the Electron Microscope
Anthea Scothern & David Garrod

The Nucleus

20 A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy (SEM)
Stephen Murray and Elena Kiseleva

21. High Pressure Freezing and Freeze Substitution of S.pombe and S.cerevisiae for TEM
Stephen Murray

22. Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans
Merav Cohen, Rachel Santarella, Naama Wiesel, Iain Mattaj, and Yosef Gruenbaum

23. Visualization of Nuclear Organisation by Ultrastructural Cytochemistry
Marco Biggiogera and Stanislav Fakan

24. Scanning Electron Microscopy of Chromosomes
Gerhard Wanner and Elizabeth Schroeder-Reiter

PART THREE Cells and Infectious Agents

25. Infection at the Cellular Level
Christian Goosmann, Ulrike Abu Abed, and Volker Brinkmann

26. Electron Microscopy of Viruses and Virus-Cell Interactions
Peter Wild
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