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Structural Biology Using Electrons and X-rays
 
 

Structural Biology Using Electrons and X-rays, 1st Edition

An Introduction for Biologists

 
Structural Biology Using Electrons and X-rays, 1st Edition,Michael F Moody,ISBN9780123705815
 
 
 

M Moody   

Academic Press

9780123705815

9780080919454

464

235 X 191

An accessible introduction to the two major diffraction-based techniques of structural biology

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Key Features

*Emphasis on common principles underlying all diffraction-based methods

*Thorough grounding in theory requires understanding of only simple algebra

*Visual representations and explanations of challenging content

*Mathematical detail offered in short-course form to parallel the text

Description

Structural Biology Using Electrons and X-Rays discusses the diffraction and image-based methods used for the determination of complex biological macromolecules. The book focuses on the Fourier transform theory, which is a mathematical function that is computed to transform signals between time and frequency domain. Composed of five parts, the book examines the development of nuclear magnetic resonance (NMR), which allows the calculation of the images of a certain protein. Parts 1 to 4 provide the basic information and the applications of Fourier transforms, as well as the different methods used for image processing using X-ray crystallography and the analysis of electron micrographs. Part 5 focuses entirely on the mathematical aspect of Fourier transforms. In addition, the book examines detailed structural analyses of a specimen’s symmetry (i.e., crystals, helices, polyhedral viruses and asymmetrical particles). This book is intended for the biologist or biochemist who is interested in different methods and techniques for calculating the images of proteins using nuclear magnetic resonance (NMR). It is also suitable for readers without a background in physical chemistry or mathematics.

Readership

Graduate and advanced undergraduate students in biochemistry, molecular biology, and biological and medical physics; research biologists using electron microscopy

Michael F Moody

Structural Biology Using Electrons and X-rays, 1st Edition

Part I: Fourier Transforms

1. Overview

2. Correlations & Convolutions

3. Fourier Fundamentals

4. Digital Fourier Transforms

5. Filters

6. Two-Dimensional FTs

Part II: Optics

7. Microscopy with Rays

8. Waves

9. Wave Imaging

Part III: Further Mathematical Methods/General Aspects

10. Symmetry

11. Statistics & Matrices

12. The Third Dimension

Part IV: Image Analysis/Structure Determination

13. Special Symmetries

14. Crystalline Sheets

15. Helices

16. Icosahedral Particles

17. Unsymmetrical ("Single") Particles

18. Distortion Correction

19. FT Mathematics

20. Elementary Matrices

 
 

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