Bioconjugate Techniques, 3rd Edition

Bioconjugate Techniques, 3rd Edition,Greg Hermanson,ISBN9780123822390


Academic Press




276 X 216

The book provides researchers proven methods and techniques for synthesizing biofunctional macromolecules and resources to engage in the fields of diagnostics, biomaterials, and pharmacotherapeutics.

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Key Features

*Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
*Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
*Features full color illustrations 
*Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented


Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.


Researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, and microbiology

Greg Hermanson

Affiliations and Expertise

Director of Technology, Thermo Fisher Scientific, Rockford, IL USA

View additional works by Greg T. Hermanson

Bioconjugate Techniques, 3rd Edition

1. Introduction to Bioconjugation

2. Functional Targets for Bioconjugation

3. The Reactions of Bioconjugation

4. Zero-Length Crosslinkers

5. Homobifunctional Crosslinkers

6. Heterobifunctional Crosslinkers

7. Trifunctional Crosslinkers

8. Dendrimers and Dendrons

9. Cross-Bridges and Cleavable Reagent Systems

10. Fluorescent Probes

11. (Strept)avidin - Biotin Systems

12. Isotopic Labeling Techniques

13. Silane Coupling Agents

14. Microparticles and Nanoparticles

15. Immobilization of Ligands on Chromatography Supports

16. Buckyballs, Fullerenes, and Carbon Nanotubes

17. Chemoselective Ligation; Bioorthogonal Reagents

18. PEGylation & Synthetic Polymer Modification

19. Vaccine and Immunogen Conjugates

20. Antibody Modification and Conjugation

21. Liposome Conjugates and Derivatives

22. Enzyme Modification and Conjugation

23. Nucleic Acid and Oligonucleotide Modification and Conjugation

24. Bioconjugation for the Study of Protein Interactions

Quotes and reviews

"Most of the chapters address specific systems and reagents such as (strept) avidin-biotin, silane coupling, buckyballs & fullerenes, antibodies, liposome conjugates, and modified nucleotides-as well as a few chapters on observation techniques such as fluorescent probes and isotopic labeling. Also new since the last edition is a chapter addressing immobilization in chromatography.", February 2014
"This is an incomparable and essential guide for any scientist involved in conjugating biologically active molecules. The new edition does not disappoint with its liberal use of color (especially in reaction schemes), expanded pages, and newly added chapters. For those interested in bioconjugate chemistry, you may soon find that this is the only book on your research library shelf!" Rating: 5, February 7, 2014

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