DNA Repair, Part A

DNA Repair, Part A, 1st Edition

DNA Repair, Part A, 1st Edition,Judith Campbell,Paul Modrich,ISBN9780121828134

Methods in Enzymology

Campbell   &   Modrich   

Academic Press




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Enzyme systems that fix DNA!

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Key Features

*A laboratory standard for more than 40 years
*Over 400 volumes strong
*Also available on ScienceDirect
*Part A of a 2-part series


This volume provides detailed coverage of modern methods for molecular analysis of enzymes and enzyme systems that function in the maintenance of genome integrity. Coverage areas include base excision repair, nucleotide excision repair, translesion DNA polymerases, mismatch repair, genetic recombination, and double strand break repair.


Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists

Judith Campbell

Affiliations and Expertise

California Institute of Technology, Pasadena, California, U.S.A.

Paul Modrich

Affiliations and Expertise

Department of Biochemistry, Duke University Medical Center, Durham, North Carolina, USA

DNA Repair, Part A, 1st Edition

Table of Contents

Chapter 1
Assays for determining lesion bypass efficiency and mutagenicity of site-specific DNA lesions in vivo
Chapter 2
Oxidative DNA glycolases: Recipes from Cloning to Characterization
Chapter 3
Purification and Characterization of NEIL1 and NEIL2, Members of a distinct Family of Mammalian DNA Glycosylases for repair of oxidized bases
Chapter 4
Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-links
Chapter 5
Isolation and analyses of MutY and MutY Homologs (MYH)
Chapter 6
Use of yeast for detection of endogenous abasic legions, their source and their repair
Chapter 7
Activities and Mechanism of Pol Beta
Chapter 8
Direct removal of aklylation damage from DNA by AlkB and related DNA dioxygenases
Chapter 9
Purification and Characterization of DNA Photolyases
Chapter 10
Genetic and in vitro assays of DNA deamination
Chapter 11
The XPC complex and UV-DDB: Functional assays for the damage recognition factors involved in global genome repair
Chapter 12
Purification and Characterization of E. coli and Human Nucleotide Excision Repair
Chapter 13
Assays for transcription elongation by RNA polymerase II using oligo (dC)-tailed
template with single DNA damage
Chapter 14
In vivo assays for transcription-coupled repair
Chapter 15
TFIIh enzymatic activities in transcription and nucleotide excision repair
Chapter 16
An assay for studying ubiquitylation of RNA polymerase II and other proteins in crude yeast extracts
Chapter 17
Analysis of the excision step in human DNA mismatch repair
Chapter 18
Characterisation of the “mismatch repairosome” and its role in the processing of modified nucleosides in vitro
Chapter 19
Analysis of DNA Mismatch Repair in Cellular Response to DNA Damage
Chapter 20
Characterization of E. coli Translesion Synthesis Polymerases and Their Accessory Factors
Chapter 21
Measuring the Fidelity of Translesion DNA Synthesis
Chapter 22
DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification and Mouse Models for Studying Their Function
Chapter 23
Purification and Characterization of Escherichia coli DNA Polymerase V
Chapter 24
Yeast and Human Translesion DNA Synthesis Polymerases: Expression, Purification, and Biochemical Characterization
Chapter 25
Localisation of Y-family polymerases and the DNA polymerase switch in mammalian cells
Chapter 26
Repair of DNA Double Strand Breaks – in vivo Biochemistry
Chapter 27
Assays for nonhomologous end-joining in extracts
Chapter 28
Purification and assays of Saccharomyces cerevisiae homologous recombination proteins
Chapter 29
Analysis of DNA recombination and repair proteins in living cells by photobleaching microscopy
Chapter 30
Synthetic Junctions as Tools to Identify and Characterize Holliday Junction Resolvases
Chapter 31
In Vitro Nonhomologous End Joining Assay
Chapter 32
The RAG and HMGB1 proteins: purification and biochemical analysis of recombination signal complexes
Chapter 33
Purification and Biochemical Characterization of ATM and MRN
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