RNA Interference, 1st Edition

RNA Interference, 1st Edition,David Engelke,John Rossi,ISBN9780080455396


Academic Press



The newest volume in the critically acclaimed laboratory standard, Methods in Enzymology

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Key Features

* One of the most highly respected publications in the field of biochemistry since 1955
* Frequently consulted, and praised by researchers and reviewers alike
* Truly an essential publication for anyone in any field of the life sciences


The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent.


Biochemists, cell biologists, molecular biologists, geneticists

David Engelke

Affiliations and Expertise

University of Michigan, Ann Arbor, U.S.A.

John Rossi

Affiliations and Expertise

City of Hope's Beckman Research Institute, Duarte, California, U.S.A.

RNA Interference, 1st Edition

Transgene-induced RNA Interference as a Tool for Plant Functional Genomics
Constructs and Methods for hairpin RNA-mediated Gene Silencing in Plants
RNA interference in Caenorhabditis elegans
High-throughput RNAi screens in Drosophila tissue culture cells
Mechanistic insights aid computational siRNA design
Novel methods for expressing RNAi in human cells
Delivery of Small Interfering RNA to Mammalian Cells in Culture Using Cationic Lipid/Polymer-Based Transfection Reagents
The subcellular distribution of siRNA: directed delivery through RNA polymerase III expression cassettes and localization using in situ hybridization
Viral delivery of recombinant shRNAs
Downregulation of cellular genes by PCR cassettes expressing short interfering RNAs
Using short hairpin RNA expression vectors to study mammalian neural development
Analysis of T-cell development using siRNA to knockdown protein expression
Viral delivery of recombinant siRNA expression: lentivirus
Paradigms for Conditional Expression of RNAi Molecules for use Against Viral Targets
High throughput RNAi strategies for target discovery and validation using synthetic siRNAs: functional genomics investigations of biological pathways
RNA interference in vivo: towards synthetic siRNA based therapeutics
Labeling and characterization of small RNAs associated with the RNAi effector complex, RITS
RNAi spreading in C. elegans
Human Dicer: Purification, properties, and interaction with PAZ PIWI domain proteins
Delivery of siRNA and siRNA Expression Constructions to Adult Mammals Using Hydrodynamic Intravascular Injection
Analysis of siRNA function in RNAi using Drosophila Embryo extracts and Schneider cells
Using RNA polymerase II to transcribe artificial microRNAs
Adeno-associated virus vectors for short hairpin RNA expression
Simple, Robust Strategies for Generating ddRNAi Constructs
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