Biophotonics, Part B

Biophotonics, Part B, 1st Edition

Biophotonics, Part B, 1st Edition,Gerard Marriott,Ian Parker,ISBN9780080497044

Marriott   &   Parker   

Academic Press



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Key Features

@introbul:Key Features
@bul:* Optical instrumentation for imaging, screening and diagnosis in molecules, tissues, and cells
* Development and application of optical probes and techniques for imaging and drug screening, protemics, genomics, and cellomics
* Applications of biophotonics research to the understanding of mechanisms of cellular reactions and processes, investigating the structure and dynamics of biomolecular systems, screening and drug discovery, and diagnosis and treatment of disease


This volume and its companion volume 360 introduce a new topic to the Methods in Enzymology series. They will cover, among other topics, imaging, screening, and diagnosis in biological systems. See key features for greater detail.


Biochemists, Biophysicists, Physiologists, Cell Biologists, Pharmacologists, Geneticists

Gerard Marriott

Affiliations and Expertise

University of Wisconsin, U.S.A.

Ian Parker

Affiliations and Expertise

University of California, Irvine

Biophotonics, Part B, 1st Edition

Total Internal Reflection Fluorescence Microscopy in Cell Biology
Reflection Interference Contrast Microscopy
Second Harmonic Imaging Microscopy
Fluorescence Correlation Spectroscopy
Fluorescence Correlation Spectroscopy of GFP Fusion Proteins in Living Plant Cells
Building and Using Optical Traps to Study Properties of Molecular Motors
Optical-Trap Force Transducer That Operates by Direct Measurement of Light Momentum
Application of Optical Traps in Vivo
Cytomechanics Applications of Optical Sectioning Microscopy
Measurements of Cell-Generated Deformations on Flexible Substrata Using Correlation-Based Optical Flow
Single-Molecule Imaging of Rotation of F1-ATPase
Molecular Motors and Single-Molecule
Visualization of Single Molecules of mRNA in Situ
Single Ion Channel Imaging
GFP-Fusion Proteins as Fluorescent Reporters to Study Organelle and Cytoskeleton Dynamics in Chemotaxis and Phagocytosis
Dynamic Imaging of Cell-Substrate Contacts
Imaging Mitochondrial Function in Intact Cells
Dynamic Imaging of Neuronal Cytoskeleton
Imaging Calcium Dynamics in Developing Neurons
Optical Monitoring of Neural Activity Using Voltage-Sensitive Dyes
Steady-State Fluorescence Imaging of Neoplasia
In Vivo and ex Vivo Tissue Applications of Two-Photon Microscopy
Fluorescence Anisotropy in Pharmacologic Screening
Surface and Printing Effects on Fluorescent Images of Immobilized Biomolecule Arrays

Quotes and reviews

"Methods in Enzymology is a highly respected, widely available, and practical series of volumes that contains detailed protocols and descriptions of biochemical and biophysical techniques...They have compiled fifty-three excellent chapters on the broad subject of biophotonics, which were written by internationally renowned scientists who work in twelve countries...The editors of Biophotonics are to be congratulated for having made a judicious selection of both the topics to be covered and also the quality of the authors they have selected to present these topics…I suggest that Biophotonics would be extremely useful to any researcher who is working in this expanding field. Undergraduate students beginning their research projects, graduate students, scientists, engineers, and clinicians involved with biophotonics would comprise the intended audience...I highly recommend Biophotonics as a very reliable and useful reference work...The very high quality illustrations are well documented with legends and annotations...The production quality of these volumes is very high...In summary, the two-volume set of Biophotnics constitutes an extensive set of protocols, techniques, instruments, and methods of analysis that constitute that state-of-the art. The books are extremely well organized, clearly written, profusely illustrated, and thoroughly documented. The editors and the diverse group of authors have succeeded in their aims and have produced a highly useful and recommended set of books."
- Journal of Biomedical Optics (2005)
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