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Sphingolipid Metabolism and Cell Signaling, Part A

Sphingolipid Metabolism and Cell Signaling, Part A, 1st Edition

Sphingolipid Metabolism and Cell Signaling, Part A, 1st Edition,Alfred Merrill, Jr.,Yusuf Hannun,ISBN9780080496689

Merrill, Jr.   &   Hannun   

Abelson  &   Simon   

Academic Press



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Sphingolipids are found in all eukaryotic and in some prokaryotic organisms and provide structure for cell membranes, lipoproteins, and other biological materials as well as participate in the regulation of cell growth, differentiation, and diverse cell functions, including cell-cell communication, cell-substratum interactions, and intracellular signal transduction. This volume presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs. Its companion Volume 312 will contain information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Biochemists, molecular biologists, cell biologists, biomedical researchers, microbiologists, and developmental biologists.

Alfred Merrill, Jr.

Affiliations and Expertise

Emory University, Atlanta, Georgia, U.S.A.

Yusuf Hannun

Affiliations and Expertise

Medical University of South Carolina, Charleston, U.S.A.

Sphingolipid Metabolism and Cell Signaling, Part A, 1st Edition

Sphingolipid Metabolism: Biosynthesis: R.C. Dickson, R.L. Lester, and M. Marek-Nagiec, Serine Palmitoyltransferase. M.M. Grilley and J. Y. Takemoto, Assay of the Saccharomyces cerevisiae Dihydrosphingosine C-4 Hydorxylase. B. Caligan, K. Peters. J. Ou, E. Wang, and A.H. Merrill, Jr., Ceramide Synthase. H. Schulze, C. Michel, and G. van Echten-Deckert, Dihydroceramide Desaturase. M.N. Nikolava-Karakashian, Assays for the Synthesis of Sphingomyelin (Ceramide Phosphocholine) and Ceramide Phosphoethanolamine. J.A. Shayman and A. Abe, Glucosylceramide Synthase: Assay and Properties. P. Marks, Y. Kamisaka, and R.E. Pagano, Methods for Studying Glucosylceramide Synthase. H. Sprong, G. van Meer, and P. van der Sluijs, UDP-Galactose: Ceramide Galactosyltransferase. S. Chatterjee, Assay of Lactosylceramide Synthase and Comments on Its Potential Role in Signal Transduction. G. Pohlentz, C. Kaes, and K. Sandhoff, In Vitro Assays for Enzymes of Ganglioside Synthesis. F.B. Jungalwala, P. Chatturvedi, D.S. Newburg, and M.R. Natowicz, Analyses of Sulfatides and Enzymes of Sulfatide Metabolism. J.A. Shayman and A. Abe, 1-O-Acyleceramide Synthase. T-C. Lee, N-Acetylation of Shingosine by Platelet-Activating Factor: Sphingosine Transacetylase. A.S. Fischl, Y. Liu, A. Browdy, and A.E. Cremesti, Inositolphosphoryl Ceramide Synthase from Yeast. D.V. Lynch, Enzymes of Shingolipid Metabolism in Plants. Turnover: S. Lansmann, O. Bartelsen, and K. Sandhoff, Purification and Characterization of Recombinant Human Acid Sphingomyelinase. B. Liu and Y.A. Hannun, Purification of Rat Brain Membrane Neutral Sphingomyelinase. B.Liu and Y.A. Hannun, Sphingolmyelinase Assay using Radiolabeled Substrates. A.G. Barbone, A.C. Jackson, D.M. Ritchie, and D.C. Argentieri, Robotic Assay of Sphingomyelinase Activity for High-Throughput Screening. D.F. Hassler, R. M. Laethem, and G.K. Smith, A High Through-put Sphingomyelinase Assay. R.T. Dobrowsky and V.G. Rao, Analysis of Sphingomyelin Hydrolosis in Caveolar Membranes. M.N. Nikolova-Karakashian and A.H. Merrill, Jr.,Ceramidases. T.Linke, S. Lansman, and K. Sandhoff, Purification of Acid Ceramidase from Human Placenta. S. Bajjalieh and R. Batchelor, Ceramide Kinase. A.Olivera, K.D. Barlow, and S. Spiegel, Assaying Sphingosine Kinase Activity. L.M. Obeid, Sphingosine 1-Phosphate Phosphatase. D.N. Brindley, J. Xu, R. Jasinska, and D.W. Waggoner, Analysis of Ceramide 1-Phosphate and Sphingosine 1-Phosphate Phosphatase Activities. P. Van Veldhover, Sphingosine 1-Phosphate Lyase. W. Bierfreund, T. Kolter, and K. Sandhoff, Sphingolipid Hydrolases and Activator Proteins. R-D. Duan and A. Nilsson, Sphingolipid Hydrolyzing Enzymes in the Gastrointestinal Tract. M. Basu, P. Kelly, M. Girzadas, Z. Li, and S. Basu, Properties of Animal Ceramide Glycanases. M. Ito, K. Kita, T. Kurita, N. Sueyoshi, and H. Izu, Enzymatic N-Deacylation of Shingolipids. Genetic Approaches: S. Ichikawa and Y. Hirabayashi, Genetic Approaches for Studies of Glycolipid Synthetic Enzymes. N.Chung and L.M. Obeid, Use of Yeast as a Model System for Studies of Sphingolipid Metabolism and Signaling. Inhibitors and Sphingolipid Biosynthesis: S.M. Mandala and G. H. Harris, Isolation and Characterization of Novel Inhibitors of Sphingolipid Synthesis: Austalifungin, Viridiofungins, Rustmicin, and Khafrefungin. R.T. Riley and R.D. Plattner, Fermentation, Partial Purification, and Use of Serine Palmitoyltransferase Inhibitors from Isaria (Cordyceps) sinclairii. F. Meredith, Isolation and Characterization of Fumonisins. J.A. Shayman, L. Lee, A. Abe, and L. Shu, Inhibitors of Glucosylceramide Synthase. Chemical and Enzymatic Syntheses: C. Cufman and D.C. Liotta, Synthesis of Sphingosine and Sphingoid Bases. K-H. Jung and R.K. Schmidt, Synthesis of Sphingosine, Radiolabeled Sphiongosine, 4-Methyl-cis-Sphingosine and 1-Amino Derivatives of Sphingosine via their Azido Derivatives. P.K. Koskinen and A.M.P. Koskinen, Total Synthesis of Sphingosine and its Analogs. A. Bielawska, Y.A. Hannun, and A. Szulc, Radiolabeled Sphingolipids, Part I. Radiolabeling in the Sphingolipid Backbone A. Bielawska and Y.A. Hannun, Radiolabeled Sphingolipids, Part II. Preparation of Radiolabeled Ceramides and Phospholsphingolipids. A. Bielawska, Z. Szulc, and Y.A. Hannun, Radiolabeled Sphingolipids, Part III. Synthesis of Key Precursors. A.S. Bushnev and D.C. Liotta, Practical Synthesis of N-Palmitoylsphingomyelin and N-Palmitoyldihydrosphingomyelin. M. Kiso and H. Ishida, Synthesis of Glycolipids, with a Focus on GM3, and Sulfatide Analogs. F. Knoll, T. Kolter, and K. Sandhoff, Sphingolipid Photoaffinity Labels. G. Schwarzmann, Synthesis and Characterization of Metabolically Stable Sphingolipids. J.Fanitni, Synthetic Soluble Analogs of Glycolipids for Studies of Virus Glycolipid Interactions. S. Sonnino, V. Chigorno, and G. Tettamanti, Preparation of Radioactive Gangliosides, 3H or 14C Isotopically Labeled at the Oligosaccharide or Ceramide Moieties. L. Riboni, P. Viani, and G. Tettamanti, Estimating Sphingolipid Metabolism and Trafficking in Cultured Cells Using Radiolabeled Compounds. M. Ito, S. Mitsutake, M. Tani, and K. Kita, Enzymatic Synthesis of 14C Ceramide, 14 C Glycosphingolipids, and W-Aminoceramide.

Quotes and reviews

@from:Praise for the Series
@qu:"The Methods in Enzymology series represents the gold-standard."
@qu:"Incomparably useful."
@qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
@qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
@qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
@qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
@qu:"A series that has established itself as a definitive reference for biochemists."
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