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Adult Stem Cells
1st Edition, Volume 419 - November 22, 2006
Editors: Irina Klimanskaya, Robert Lanza
Language: English
Hardback ISBN:9780123736505
9 7 8 - 0 - 1 2 - 3 7 3 6 5 0 - 5
eBook ISBN:9780080469164
9 7 8 - 0 - 0 8 - 0 4 6 9 1 6 - 4
This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusi…Read more
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This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook” and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional” and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.
Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering
Presents the latest most innovative technologies
Addresses therapeutic relevance including FDA compliance and tissue engineering
Biochemists, cell and molecular biologists, and bioengineers working with stem cells.
Dedication
Contributors to Volume 419
Preface
Foreword
Contents of Previous Volumes
Section I: Ectoderm
[1]. Neural Stem Cell Isolation and Characterization
Abstract
Introduction
Reagents and Instrumentation
Methods
Acknowledgments
[2]. Neural Stem Cells and Their Manipulation
Abstract
Introduction
Adult Niches for Stem Cells In Vivo
In Vitro Manipulation of NSCs
Conclusions and Projections
Sample Protocols for the Culture and Characterization of NSCs
Acknowledgments
[3]. Retinal Stem Cells
Abstract
Introduction
Materials and Methods
Overview
[4]. Epithelial Skin Stem Cells
Abstract
Introduction to Skin Epithelial Stem Cells
Epithelial Skin Organization and the Hair Cycle
Characteristics of Skin Epithelial Stem Cells
[5]. Dental Pulp Stem Cells
Abstract
Introduction
Identification of Dental Pulp Stem Cells
Isolation of DPSCs
Differentiation of DPSCs
Section II: Mesoderm
[6]. Postnatal Skeletal Stem Cells
Abstract
Introduction
Skeletal Stem Cells and Mesenchymal Stem Cells
Defining a Skeletal Stem Cell
Origin and Nature of Skeletal Stem Cells
Plasticity of BMSCs
Role of Skeletal Stem Cells in Disease
Molecular Engineering
Potential Use of Skeletal Stem Cells in Tissue Engineering and Regenerative Medicine
Establishment of Nonclonal Populations of BMSCs In Vitro
Establishment of Clonal Populations of BMSCs In Vitro
Determination of Colony-Forming Efficiency
In Vitro Differentiation of BMSCs
In Vivo Differentiation of BMSCs
Acknowledgments
[7]. Hematopoietic Stem Cells
Abstract
Introduction
Identification and Enrichment of HSCs
Functional Characterization of Candidate HSCs
Long-Term Culture of Candidate HSCs and Progenitors
Acknowledgments
[8]. Hemangioblasts and Their Progeny
Abstract
Introduction
Ex Vivo Expansion of CD133+ Cells
Hematopoietic and Endothelial Differentiation of Single Cell-Derived EGFP+ Clones
Phenotypical and Functional Studies of CD133-Derived Cells during Ex Vivo Expansion and Differentiation
Materials
Reagents
Methods
[9]. Kidney Epithelial Cells
Abstract
Introduction
Analysis of Steady State Tubular Epithelium Maintenance
Regeneration of Experimentally Injured Kidney Tubules
Extratubular Progenitor Cells
Culture of Human Tubular Progenitor Cells
[10]. Ovarian Germ Cells
Abstract
Introduction
Origin of Primordial Germ Cells
Formation of Human Fetal Oocytes and Primordial and Primary Follicles
Immune System-Related Cells and Oogenesis in Fetal Ovaries
Fetal Programming of Follicular Renewal during Adulthood
“Ovary within the Ovary” Pattern and Thy-1 Differentiation Protein
Cessation of Adult Oogenesis
Oogenesis and Follicular Renewal in Adult Ovaries
Working Hypothesis on the Role of the Gonadal Environment
Differentiation of Stem Cells and Tissue Control System Theory
Potential Treatment of Ovarian Infertility by Producing New Primary Follicles In Vivo
Mystery of the Origin of Oocytes in Adult Mammalian Ovaries
Development of Distinct Cell Types from Totipotent Ovarian Stem Cells In Vitro
Ovarian Stem Cell Culture Protocol
Ovarian Stem Cell Cultures and Autologous Treatment of POF
Potential Pitfalls
Autologous Ovarian Stem Cells and Treatment of Degenerative Diseases
Conclusion
Acknowledgment
[11]. Spermatogonial Stem Cells
Abstract
Introduction
Spermatogonial Stem Cells
SSC Transplantation
SSC Culture
Implications
Acknowledgments
Section III: Endoderm
[12]. Stem Cells in the Lung
Abstract
Introduction
Anatomical and Cellular Diversity of Adult Lung
Stem Cell Phenotypes and Niches in Adult Lung
In Vivo Injury Models of the Lung
Ex Vivo Epithelial Tracheal Xenograft Model to Study Stem Cell Expansion in Proximal Airway
In Vitro Colony-Forming Efficiency Assay to Characterize Stem Cell Populations in Conducting Airway Epithelium
Models to Study Stem/Progenitor Cells of Airway Submucosal Glands
Acknowledgments
[13]. Pancreatic Cells and Their Progenitors
Abstract
Introduction
Pancreas Development
Origins of Beta Cells During Postnatal Life
Summary and Perspective
Methods: Design of a Lineage-Tracing Experiment in Mice
[14]. Intestinal Epithelial Stem Cells and Progenitors
Abstract
Introduction
Assaying Intestinal Stem Cells, Progenitors, and Their Lineages In Vivo
Intestinal Epithelium In Vitro
Section IV: Extraembryonic and Perinatal Stem Cells
[15]. Trophoblast Stem Cells
Abstract
Introduction
Preparations for Culture of TS Cells
Preparation of Feeder Cell Stocks
Establishment of TS Cell Lines
Passage and Maintenance of TS Cells
Freezing and Thawing of TS Cells
[16]. Pluripotent Stem Cells from Germ Cells
Abstract
Introduction
Germ Cell Development
Embryonic Germ Cell Derivation
Characterization of EG Cultures
Embryoid Body Formation and Analysis
Embryoid Body-Derived Cells
Acknowledgments
[17]. Amniotic Fluid and Placental Stem Cells
Abstract
Introduction
Amniotic Fluid and Placenta in Developmental Biology
Amniotic Fluid and Placenta for Cell Therapy
Isolation and Characterization of Progenitor Cells
Differentiation of Amniotic Fluid- and Placenta-Derived Progenitor Cells
Amniotic Fluid and Placental Differentiation Protocols
Conclusion
[18]. Cord Blood Stem and Progenitor Cells
Abstract
Introduction: Cord Blood Transplantation
Hematopoietic Stem and Progenitor Cells
Endothelial Progenitor Cells
Methodologies for Assessing Hematopoietic Progenitor and Stem Cells, as Well as Endothelial Progenitor Cells, Present in Human Cord Blood
Methods for Enumerating Human Myeloid Progenitor Cells in Umbilical Cord Blood by In Vitro Colony Formation
Methods for Enumerating Human Hematopoietic Stem Cells in Umbilical Cord Blood
Methods for Enumerating Endothelial Progenitor Cells in Umbilical Cord Blood
Uses of Preceding Assays
Author Index
Subject Index
No. of pages: 576
Language: English
Edition: 1
Volume: 419
Published: November 22, 2006
Imprint: Academic Press
Hardback ISBN: 9780123736505
eBook ISBN: 9780080469164
IK
Irina Klimanskaya
Affiliations and expertise
Advance Cell Technology, Worcester, MA, USA
RL
Robert Lanza
Robert Lanza is an American scientist and author whose research spans the range of natural science, from biology to theoretical physics. TIME magazine recognized him as one of the “100 Most Influential People in the World,” and Prospect magazine named him one of the Top 50 “World Thinkers.” He has hundreds of scientific publications and over 30 books, including definitive references in the fields of stem cells, tissue engineering, and regenerative medicine. He’s a former Fulbright Scholar and studied with polio-pioneer Jonas Salk and Nobel laureates Gerald Edelman (known for his work on the biological basis of consciousness) and Rodney Porter. He also worked closely (and co-authored papers in Science on self-awareness and symbolic communication) with noted Harvard psychologist BF Skinner. Dr. Lanza was part of the team that cloned the world’s first human embryo, the first endangered species, and published the first-ever reports of pluripotent stem cell use in humans.
Affiliations and expertise
Astellas Institute for Regenerative Medicine, Westborough, MA, USA