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Cilia: Structure and Motility
1st Edition, Volume 91 - November 27, 2009
Editors: Stephen M. King, Gregory J Pazour
Language: English
Hardback ISBN:9780123749734
9 7 8 - 0 - 1 2 - 3 7 4 9 7 3 - 4
eBook ISBN:9780080961064
9 7 8 - 0 - 0 8 - 0 9 6 1 0 6 - 4
Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and…Read more
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Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.
Covers protocols for cilia and flagella across systems and species
Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time
Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures
Researchers and students in cell, molecular and developmental biology
1. Cryo-Electron Microscope Tomography to Study Axonemal Organization Daniela Nicastro
2. Electron Microscopic Imaging and Analysis of Isolated Dynein Particles Anthony J. Roberts and Stan A. Burgess
3. Immunogold Labeling of Flagellar Components In Situ Stefan Geimer
4. Scanning Electron Microscopy to Examine Cells and Organs Jovenal T. SanAgustin, John A. Follit, Gregory Hendricks, and Gregory J. Pazour
5. X-ray Fiber Diffraction Studies on Flagellar Axonemes Kazuhiro Oiwa, Shinji Kamimura, and Hiroyuki Iwamoto
6. Markers for Neuronal Cilia Jacqueline S. Domire and Kirk Mykytyn
7. Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells Heymut Omran and Niki T. Loges
8. Immunoprecipitation to Examine Protein Complexes Gregory J. Pazour
9. Tandem Affinity Purification of Ciliopathy-Associated Protein Complexes Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner, Marius Ueffing, and Ronald Roepman
10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products Miho Sakato
11. Analysis of the Ciliary/Flagellar Beating of Chlamydomonas Kenneth W. Foster
12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii Ritsu Kamiya
13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain Karl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman
14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow Yasushi Okada and Nobutaka Hirokawa
15. Modification of Mouse Nodal Flow by Applying Artificial Flow Shigenori Nonaka
16. Measuring Cilium-Induced Ca2þ Increases in Cultured Renal Epithelia Helle A. Praetorius
No. of pages: 344
Language: English
Edition: 1
Volume: 91
Published: November 27, 2009
Imprint: Academic Press
Hardback ISBN: 9780123749734
eBook ISBN: 9780080961064
SK
Stephen M. King
Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.
Affiliations and expertise
Professor, Department of Molecular Biology and Biophysics Director, Electron Microscopy Facility, University of Connecticut Health Center