Biomedical Optical Phase Microscopy and Nanoscopy

Biomedical Optical Phase Microscopy and Nanoscopy, 1st Edition

Biomedical Optical Phase Microscopy and Nanoscopy, 1st Edition,Natan T. Shaked,Zeev Zalevsky,Lisa Satterwhite,ISBN9780124158719

Shaked   &   Zalevsky   &   Satterwhite   

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Key Features

  • Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications.
  • Tips/advice on building systems and working with advanced imaging biomedical techniques, including  interpretation of phase images, and techniques for quantitative analysis based on phase microscopy.
  • Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.


Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy

The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications.


Biomedical engineers and researchers
Nanotechnology engineers and researchers
Optical engineers and researchers

Microscopy engineers

Natan T. Shaked

Affiliations and Expertise

Senior Lecturer, Department of Biomedical Engineering, Tel Aviv University

Zeev Zalevsky

Affiliations and Expertise

Faculty of Engineering and the Center for Advanced Materials and Nanotechnology, Bar-Ilan University

View additional works by Zeev Zalevsky

Lisa Satterwhite

Affiliations and Expertise

Duke University, Durham

Biomedical Optical Phase Microscopy and Nanoscopy, 1st Edition

Natan T. Shaked, Zeev Zalevskey and Lisa L. Satterwhite

  1. Phase contrast microscopy and differential interference contrast microscopy
  2. Chapter 1

    Principles of phase contrast microscopy

    Sam A. Johnson

    Editor: Lisa L. Satterwhite

    Chapter 2

    Differential interference contrast (DIC) microscopy

    Michael Shribak
    Editor: Lisa L. Satterwhite

    Chapter 3

    Long term phase contrast imaging of untransformed human cells

    Yumi Uetake and Greenfield Sluder

    Editor: Lisa L. Satterwhite

    Chapter 4

    Phase imaging of plant cells and tissues

    Vassilios Sarfis
    Editor: Zeev Zalevsky

  3. Digital holographic phase microscopy
  4. Chapter 5

    Measuring biophysical parameters of living cells with digital holographic microscopy

    Benjamin Rappaz, Christian Depeursinge and Pierre Marquet

    Editor: Natan T. Shaked

    Chapter 6

    New digital holographic microscopy setups and techniques for obtaining multi-focus quantitative phase imaging of living cells

    Björn Kemper, Patrik Langehanenberg and Andreas Bauwens

    Editor: Natan T. Shaked

    Chapter 7

    Phase unwrapping problems in digital holographic microscopy of biological cells
    Alexander Khmaladze

    Editor: Natan T. Shaked

    Chapter 8

    Partially-coherent lensless holographic microscopy on a chip and its application for automated semen analysis

    Ting-Wei Su and Aydogan Ozcan

    Editor: Natan T. Shaked

    Chapter 9

    Synthetic aperture lensless digital holographic microscopy for superresolved biological imaging

    Vicente Micó, Zeev Zalevsky, Luis Granero and Javier García

    Editor: Zeev Zalevsky

    Chapter 10

    Combining digital holographic microscopy with microfluidics: a new tool in biotechnology.

    Lisa Miccio, Pasquale Memmolo, Fraancesco Merola, Melania Paturzo and Pietro Ferraro

    Editor: Zeev Zalevsky

    Chapter 11

    Holographic motility contrast imaging of live tissues

    David D. Nolte, Ran An, Kwan Jeong and John Turek

    Editor: Natan T. Shaked


  5. Advanced interferometric and polarization techniques
  6. Chapter 12

    Tomographic phase microscopy

    Wonshik Choi

    Editor: Natan T. Shaked

    Chapter 13

    Phase-sensitive optical coherence tomography

    Itay Shock and Natan T. Shaked

    Editor: Natan T. Shaked

    Chapter 14

    Polarization and spectral interferometric techniques for quantitative phase microscopy

    Yizheng Zhu, Matthew T. Rinehart, Francisco E. Robles and Adam Wax

    Editor: Natan T. Shaked


    Chapter 15

    Polarization microscopy
    Rudolf Oldenbourg
    Editor: Lisa L. Satterwhite


  7. Phase nanoscopy

Chapter 16

Is there a fundamental limit to optical resolution in phase microscopy?
Steve Lipson

Editor: Zeev Zalevsky

Chapter 17

Nano-holographic interferometry for in-vivo observations
Federico Sciammarella, Cesar A. Sciammarella and Luciano Lamberti

Editor: Natan T. Shaked

Chapter 18

Fluorescence phase microscopy
Alberto Bilenca, Brett Bouma, Guillermo Tearney, Iwan Märki, Noelia Bocchio,

Stefan Geissbuehler and Theo Lasser

Editor: Natan T. Shaked

Quotes and reviews

"This interesting and inspiring book is a collection of articles on specific applications in optical imaging of cells and tissues…Phase imaging is understood in a broad sense and related techniques of super-resolution, polarization and optical coherence microscopy are described in a concise but very informative manner."--Optics & Photonics News Online, June 13, 2013

"This book has been structured to be self-contained and therefore useful for researchers and students at all levels who need a cutting edge review of techniques and applications, particularly biological and medical applications."--Reference and Research Book News, December 2012

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